Home | Articles
Published on:August 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(3s):s380-s386
Original Article | doi:10.5530/ijper.53.3s.109

Method Development and Validation for Quantitative Estimation of Calcium Acetate in Calcium Acetate Capsules by RP-HPLC using Indirect UV Method

Authors and affiliation (s):

Rajesh Kumar Chawla*, Subhranshu Panda, Kulandaivelu Umasankar, Siva Prasad Panda, Dalu Damayanthi

K. L. College of Pharmacy, K. L. University, Vaddeswaram, Guntur, Andhra Pradesh, INDIA.


Objective: A simple, accurate and economical method has been developed by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) using indirect UV (Ultra Violet) method. The Principle behind the indirect UV method was applied; the copper sulfate pentahydrate with UV absorption in the mobile phase was replaced with calcium ions without UV absorption, resulting in a reduction in UV absorption of the mobile phase. Materials and Methods: Method development was carried out on strong cation exchanger column (Zorbax 300-SCX, 4.6 mm i.d. x 150 mm, particle size 5 μm). The mobile phase consists of 4 mM copper sulfate pentahydrate solution at a flow rate of 2.0 mL per min. Indirect UV detection was performed at 230.0 nm. Results: Retention time for calcium was found about 7.1 min. The developed method was validated in terms of specificity, linearity, accuracy, precision, range, limit of detection, limit of quantification and robustness. Calibration plot was linear over the concentration range of 19 to 1125 µg/mL. System suitability parameters were found 0.8 % RSD, tailing factor 1.1 and more than 8000 theoretical plates. Limit of detection and limit of quantification were found 6.3 µg/mL and 19.0 µg/mL respectively. Conclusion: The high recovery and low relative standard deviation confirm the suitability of the method for determination of calcium acetate in calcium acetate capsules.

Key words: Calcium Acetate, RP-HPLC, Indirect UV method, Chromophore, Validation.



Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).


Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

User login

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs