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Published on:July 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(3):503-510
Original Article | doi:10.5530/ijper.53.3.82

Punica granatum and Citrillus colocynthis Aqueous Extracts Protect Mice from LPS-Induced Lung Inflammation and Inhibit Metalloproteinases-2 and -9

Authors and affiliation (s):

Fatma Zioud1,2, Viviana Marzaioli3, Jamel El-Benna3,4, Rafik Bachoual1,2,*

1Faculty of Sciences of Gabes, University of Gabes, TUNISIA.

2Laboratory of Plant Improvement and Valorization of Agroresources, National School of Engineering of Sfax, Sfax, TUNISIA.

3INSERM U1149, CNRS ERL8252 Inflammation Research Center, Paris, FRANCE.

4University of Paris Diderot, Sorbonne Paris City, Inflamex Laboratories, Faculty of Medicine, Xavier Bichat, Paris, FRANCE.


Aim: Plants used in traditional medicine produce diverse active metabolites exhibiting various medicinal properties. For several centuries Citrillus colocynthis and Punica granatum have been used to treat inflammation, cancer, edema, bacterial infections and diabetes. The objectives of this work were to study the effect of C. colocynthis and P. granatum peel aqueous extracts, on activity of metalloproteinases, enzymes implicated in chronic inflammatory diseases such Chronic Obstructive Pulmonary Disease (COPD) and emphysema. Materials and Methods: Lung inflammation was induced in the mice (Strain BALB/c) by intra-tracheal instillation of lipopolysaccharide (5 μg/mouse) and P. granatum peel extract (PGE) or C. colocynthis peel extract (CCPAE) (200 mg/Kg body weight) was injected intraperitoneally prior to LPS administration. Bronchoalveolar lavage and lung tissue were collected to assess inflammatory cells count and total protein content. Metalloproteinases activity and expression was detected by RT-PCT and zymography techniques, respectively. Results: Results showed that PGE and CCPE decreased markedly neutrophils count and proteins leakage into Bronchoalveolar Lavage Fluid (BALF). Gelatin zymography assay showed that PGE inhibited MMP-2 and MMP-9 activity in BALF and in lung homogenates. However, CCPE inhibited MMP-2 and MMP- 9 in lung homogenates but only MMP-2 in BALF. Furthermore, both PGE and CCPAE inhibit MMP-2 and -9, expression in the lung. Conclusion: Our results showed that PGE and CCPAE exhibit anti-inflammatory and, antimetalloproteinases especially MMP-2 and -9, activities. According to these findings, these natural products could be used as a potential source of new compounds with anti-inflammatory and anti-metalloproteinases activity.

Key words: Lung inflammation, COPD, Emphysema, MMP-2, MMP-9, Mice.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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