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Published on:August 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(3s):s433-s436
Original Article | doi:10.5530/ijper.53.3s.116

Pharmacological Modulation of Flavivirus Methyltransferase Activity of Dengue Virus (DEV)


Authors and affiliation (s):

Afaf Salim Alwabli1,*, Sana Ghazi Alattas1, Alawiah Mohammad Alhebshi1, Nidal Mohammed Zabermawi1, Fatima Kaneez2, Esam Ibraheem Azhar3, Naseer Alkenani1, Khalid Al-Ghmady1, Ishtiaq Qadri1

1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, SAUDI ARABIA.

2Department of Applied Biochemistry, Faculty of Science, University of Jeddah, Jeddah, SAUDI ARABIA.

3Special Infectious Agents Unit, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, SAUDI ARABIA.

Abstract:

Background: The occurrence of dengue fever plagues has expanded significantly in the course of the most recent couple of decades. Be that as it may, no immunization or antiviral treatments are accessible. In this manner, the requirement for protected and successful antiviral medications have turned out to be essential. Aim: Methyltransferases, with their altered enzymatic action, have been found associated with several diseases or infections. It has been observed that the dengue virus infection is correlated with the hyperactivity of methyltransferase. In the current study, we proposed to investigate the inhibitory effects of commercially available compounds on the methyltransferase activity. Materials and Methods: Evaluation of methyltransferase activity of the purified enzyme and their inhibitors was carried out using Methyltransferase Activity Assay Kit (Colorimetric). Results: Among the screened components, we identify two effective compounds that revealed significant effect against flavivirus methyltransferase activity. Further, time-dependent inhibition profiles for both compounds revealed that preincubation of fractions of Azacitidine and Zebularine for different time windows showed time-dependent inactivation of methyltransferase as the degree of inhibition increased with time. Conclusion: We conclude that both Azacitidine and Zebularine may be emerging as possible suitable drugs for the treatment of dengue virus infection.

Key words: Methyltransferase, Dengue virus, Inhibitor, Concentration, Time course.

 

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IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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