Home | Articles
Published on:December 2019
Indian Journal of Pharmaceutical Education and Research, 2020; 54(1):85-94
Original Article | doi:10.5530/ijper.54.1.10

Antiproliferative Effect of Crude Venom from Conus virgo on Human Lung Cancer Cell Line and Toxicity Assessment on Adult Zebra Fish (Danio rerio)


Authors and affiliation (s):

Dhana Sekaran Ganesan, Mohamed Asik Raj Mohamed, Pema Lhanzin, Kathirvel Neelan, Lakshmanan Vadakkuvaselvi, Sellamuthu Subramani, Arulvasu Chinnasamy*

Unit of Animal Tissue Culture and Aquaculture, Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamilnadu, INDIA.

Abstract:

Background: For the past two decades, few conotoxins entered in clinical trial and some are available for pain relieving as well as for treatment of neurological disorders. Aim: The present investigation was made to elucidate the antiproliferative effect of crude venom from Conus virgo against human lung adenocarcinoma cells. Acute toxicity was determined on adult Zebra fish. Methods: Cytotoxicity was assessed by MTT assay where the DNA damage was carried out by DNA fragmentation method and the in vivo toxicity was assessed by OECD guidelines. Results: The cell viability was found to decrease and the cell lost their morphological integrity with increasing concentration (10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 μg/ml) with an IC50 concentration of 74.69 μg/ml. The nuclear integrity and DNA fragmentation assay revealed the induction of DNA damage which was observed at 5 h. Further acute toxicity study was made, the LD50 was found at a concentration of 96.393μg/ml. The crude venom affects the locomotory activity of adult Zebra fish and induces the paralytic activity at 50, 75, 100 and 125 μg/ml concentrations. Histology of brain tissue indicated slight variations in the pallium. Conclusion: From the investigation, it was inferred that C. virgo venom could be a potential candidate for cancer chemotherapy.

Key words: A549, Conus virgo, Cone snail, Zebra fish, Acute toxicity.

 




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the Latest Release of Journal Citation Reports (source: Web of Science Data).

 

Impact Factor® as reported in the 2023 Journal Citation Reports® (Clarivate Analytics, 2023): 0.8

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs