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Published on:November 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(4s):s607-s618
Original Article | doi:10.5530/ijper.53.4s.156

Screening and Identification of Plant Metabolites against Snake Venom Enzymes using in vitro to in silico Approach


Authors and affiliation (s):

Pushpendra Singh, Mohammad Yasir, Rahul Shrivastava*

Department of Biological Science and Engineering, Maulana Azad National Institute of Technology, Bhopal, INDIA.

Abstract:

Aim/Background: Snakebites are generally a neglected tropical disease that have harmful impact on thousands of people globally. Although antiserum therapy is the only management available for snakebites, several side effects of this therapy have raised the requirement of an alternative to treat snakebite or boost antiserum efficacy. The present study aimed to provide a scientific explanation for the use of plants extracts in neutralizing snake venom enzymes phospholipase A2, hyaluronidase, DNase and RNase. Materials and Methods: Twelve plant species were selected, which were traditionally used in tribal region of Madhya Pradesh to manage snakebites. Ethanolic and aqueous extracts of these plants were tested for in vitro enzyme neutralization. Further chemical investigation of Dryopteris cochleata rhizome was carried out followed by Molecular docking studies using Glide software tools. Results: D. cochleata extract showed the highest enzyme-neutralizing activity when compared to other plants extracts and exhibited significant antioxidant activity. Molecular docking studies of GC-MS identified that constituent 12-phenyl-2,3,7,8-tetramethoxy-5H-(1)-benzopyrano[4,3-c]isoquinoline had a Glide score of –7.455 and formed hydrogen bonds with ASP 49 amino acid residue of phospholipase A2 molecule. Conclusion: The study concludes that extract of D. cochleata rhizomes inhibit activity of toxic enzymes of snake venom.

Key words: Phospholipase A2, Hyaluronidase, Deoxyribonuclease, Ribonuclease, Dryopteris cochleata.

 

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IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs