Development of Non-invasive Electroencephalography Technique in Animal Model

Abstract:

Aim: This paper describes about the new non-invasive method to measure brain waves from the scalp of the rats. The method allows to record good quality Electroencephalogram (EEG) from freely moving rats in the cage. Methods: This experiment targeting rodents used simple adhesive patch containing conductive gel placed on the scalp of the rats has a potential to measure the brain waves without any surgical intervention. Stable electrode gel shown to have good conductivity and strongly bonds to the skin of the animal and is well suited for recording from awaken and freely moving rats. We used two channel data acquisition system to convert analogue to digital signals of the brain waves. This study conducted to record the accurate electroencephalograph signals, a helpful tool for the translational research. The major advantages of the non-invasive EEG recording in contrast to the invasive method we presented here an efficacious, quick and efficient technique for the placement of the EEG leads in rats. Results: Quality EEG data obtained using two channel data acquisition system and is used for analysis. Reliable estimation of the electrical activity of the brain from conscious rat can be made. In conventional procedures electrodes are implanted in the skull with an additional stressful procedure. Conclusion: The results provided evidence that this method can be used as an alternative to the conventional recording of the electroencephalogram. This tool definitely a valuable tool in basic research, neurodegenerative and epilepsy research.

Key words: Electroencephalogram, Fast Fourier Transform, Stereotaxis equipment, Frequency bands, Conductive gel, Absolute Power.