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Published on:December 2019
Indian Journal of Pharmaceutical Education and Research, 2020; 54(1):135-142
Original Article | doi:10.5530/ijper.54.1.16

Preliminary Assessment of Acute and 28-Day Repeated Dose Oral Toxicity of a Newly Developed Herbal Mixture on Experimental Animal


Authors and affiliation (s):

Soumendra Darbar1,2, Srimoyee Saha3, Kausikisankar Pramanik2, Atiskumar Chattopadhyay1,*

1Faculty of Science, Jadavpur University, Raja SC Mallick Road, Kolkata, West Bengal, INDIA.

2Department of Chemistry, Jadavpur University, Raja SC Mallick Road, Kolkata, West Bengal, INDIA.

3Department of Physics, Jadavpur University, Raja SC Mallick Road, Kolkata, West Bengal, INDIA.

Abstract:

Objectives: Toxicity is an expression of being poisonous, indicating the state of adverse effects led by the interaction between toxicants and cells. The present study was designed to evaluate the acute oral toxicity study and 28 days repeated toxicity study of Herbal Mixture (HM) according to OECD guidelines. Materials and Methods: In acute oral toxicity study, Herbal mixture was administered at 2000mg/kg orally and animals were observed for toxic signs at 30 min, 1, 2 and 4 hr and thereafter once a day for the next 14 days. In repeated dose-28-day toxicity study, the animals were divided into four groups of 6 animals each. Group-1 animals served as a control. Group II Animals received low dose of test drug 100 mg/kg (orally). Group III animals received middle dose of test drug 200 mg/kg (orally) once daily for 28 days respectively. Group IV animals received high dose of test drug 400 mg/kg (orally) once daily for 28 days respectively. Results: The study results showed that neither the acute toxicity study of herbal mixture at the dose level of 2000mg/kg nor the repeated dose study did not produce any toxic sign or mortality during study. In repeated dose toxicity study, no significant changes were observed in the haematological and biochemical parameters, relative organ weight, gross necropsy and histopathological examination with herbal mixture treatment. Conclusion: The results of the present study suggest that LD50 of newly developed Herbal Mixture (HM)>2000mg/kg and the mixture is completely safe and non-toxic for therapy.

Key words: Acute oral toxicity, Sub acute toxicity, Herbal mixture, Haematology, Liver function test, Histology.

 

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IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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