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Published on:May 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(2s):s182-s188
Original Article | doi:10.5530/ijper.54.2s.74

Synthesis and Characterization of Un-encapsulated and Pterostilbene-encapsulated DOTAP: Cholesterol Liposomes


Authors and affiliation (s):

Kaustubh Sunil Hiray, Suresh Palamadai Krishnan*

Department of Biomedical Sciences, School of Biosciences and Technology, VIT, Vellore, Tamil Nadu, INDIA.

Abstract:

Aim: Our study focuses on the liposome-based nanoformulation, which can encapsulate Pterostilbene for its subsequent testing in relevant, model systems for cancer. Background: Pterostilbene, a plant-derived, hydrophobic, dietary stilbenoid, has been studied for its ability to induce cell death and regulate caspases in the different types of cancer cells. The potential of this drug can be improved by formulating a suitable vehicle for its delivery. Biocompatible, lipid-based nanoparticles called liposomes have been studied as a potent delivery vehicle for drugs in pre-clinical as well as in clinical studies. Liposomes can improve the drug uptake and bioavailability of the drugs. Materials and Methods: Pterostilbene loaded liposomes were constructed using DOTAP and Cholesterol, by the Thin-Film Hydration method. Along with the loaded liposomes, blank liposomes (only DOTAP and Cholesterol, without Pterostilbene) were also constructed. The liposomes were characterized for their size, Polydispersity Index (PDI) and Zeta potential using DLS. Shape of the liposomes was analysed using TEM. Encapsulation Efficiency (EE) of the Pterostilbene loaded liposomes was determined. Also, UV-Vis spectrophotometer was used to ensure that Pterostilbene was encapsulated inside the liposomes and there was no interaction between the drug and the lipids. Results: Liposomes were composed of DOTAP and Cholesterol with molar ratios 2:1. The DLS showed that the size of the Pterostilbene-loaded liposomes was 435.6±5 nm ( n =3), PDI was 0.5±0.07 ( n =3) and Zeta potential was -16.4±0.5 mV ( n =3). The drug encapsulation efficiency was found to be 97.5±0.8% ( n =3). Conclusion: Reproducibility in the results (DLS and EE data for Pterostilbene-encapsulated liposomes) provides a sound, scientific basis for evaluating their cell death potential of Pterostilbene loaded liposomes against cancer cells in comparison with that of free Pterostilbene (parent compound). Also, the experimental flow of ours can be used as a teaching tool by educators in drug delivery and allied fields.

Key words: Pterostilbene, DOTAP, Cholesterol, Liposomes, Nanoparticles.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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