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Published on:May 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(2s):s309-s315
Original Article | doi:10.5530/ijper.54.2s.88

New Bioactive Molecules Isolated for the First Time from Hyoscyamus albus L. and their Mechanisms Underlying the Anticancer Effects


Authors and affiliation (s):

Massinissa Yahia1,2,*, Afaf Benhouda2, Mouloud Yahia2, Hamada Haba2, Francisco Campos3, Souhila Benbia2, Antonio Rangel3

1Department of Biology of Living Organisms, University of BATNA-2-, ALGERIA.

2Departments of Pharmacy, University of Naples Federico II, 80131 Naples, ITALY.

3Catholic University of Spain, Pharmacy Department Madrid, Murcia, SPAIN.

Abstract:

Background: Hyoscyamus albus L. is a small genus from Solanaceae family known by its use in old traditional medicine in the east of Algeria. Aim: This study aimed to characterize new bioactive molecules from H. albus, evaluate their anticancer activity in several cancer cells and investigate their possible molecular mechanism. Materials and Methods: New compounds (Peak h of fraction F), (Peak 3 of Fraction F), (Peak 1 of fraction C) were isolated from H.albus L byusinghigh-performance chromatography (HPLC), mass spectrometry (MS) and proton NMR (NMR H1). All isolated compounds were subjected to cytotoxicity and antiproliferative assays against a panel of the four cell lines:DU-145, U-2 OS, U-87 MG and LN-229 cell lines and was determined using MTT assay, Annexin V and propodium iodide was used to evaluate apoptosis. Results: The phytochemical study of H. albus Fractions led to the isolation of quercetin-3-O-β-dglucopyranosyl-( 1 → 6)-β-d-glucopyranosid, N-trans-feruloyltyramine, Hydrocaffeoyl-N8- caffeoylspermidine.The biological results indicated that all cell lines were consistently sensitive to P1 FC in a dose-dependent manner. This difference in cytotoxic sensitivity was more pronounced in osteosarcoma cell line, U-2 OS when compared to prostate cancer and U-87 MG. Cell viability data also demonstrated that only U-87 MG cells were responsive to treatment with Ph FF. compounds P1 FC and Ph FF have induced necrosis and apoptosis in large part of LN-229 cells. Conclusion: The overall results of the present study provided evidence that isolated compounds are potential therapeutic entities against cancer.

Key words: Hyoscyamus albus L., Solanaceae, HPLC, Cytotoxic activity, HAMeOH, NMR H1, Annexine V, Apoptosis.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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