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Published on:August 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(3):668-673
Original Article | doi:10.5530/ijper.54.3.116

Apigetrin Inhibits Thyroid Cancer Cell Growth and Proliferation through Down-Regualtion of HIF1α and VEGF Expression


Authors and affiliation (s):

Zhihua Guo1, Yuan Xing2, Wei Wang1, Jingbo Lai3,*

1Department of Otorhinolaryngology Head and Neck Surgery, Tangdu Hospital Affiliated to Air Force Military Medical University, Baqiao, Xi’an, Shaanxi, CHINA.

2Department of Otorhinolaryngology Head and Neck Surgery, Xi’an Jiaotong University School of Medicine, Honghui Hospital, Beilin, Xi’an, Shaanxi, CHINA.

3Department of Endocrinology and Metabolism, Xijing Hospital Affiliated to Air Force Military Medical University, Xincheng, Xi’an, Shaanxi, CHINA.

Abstract:

Aim: Thyroid cancer is a commonly detected endocrine malignant tumour which accounts for 1% of the total human cancers. The present study was aimed to investigate the effect of apigetrin on anaplastic thyroid cancer cell viability and understand the mechanism involved. Materials and Methods: Fluorescent microscopy using diamidino-2- phenylindole (DAPI)staining was used to examine the nuclear fragmentation. Changes in protein expression were analysed by Western blot analysis and migration potential using wound healing assay. Results: Treatment with apigetrin exhibited inhibitory effect on anaplastic thyroid cancer cell viability in dose based manner. The cleavage of PARP and activation of caspase-3/-9 was promoted in anaplastic thyroid cancer cells on treatment with apigetrin. A significant reduction in hypoxiainducible factor1α (HIF1α) and vascular endothelial growth factor (VEGF) expression was caused by apigetrin in anaplastic thyroid cancer cells. The count of Terminal deoxynucleotidyltransferase-mediated nick end labeling (TUNEL)-positive cells was increased significantly (p<0.05) in the cell cultures on treatment with apigetrin. The cell migration was also suppressed by apigetrin in dose based manner. Moreover, treatment of mice with apigetrin inhibited the tumor growth and development markedly in comparison to the untreated group. Conclusion: The present study demonstrates that apigetrin plays an important role in the inhibition of thyroid cancer. Therefore, apigetrin needs to be investigated further as therapeutic agent for thyroid cancer treatment.

Keywords: Scutellaria, Papillary, Follicular, Terpenoid, Pharmacophore.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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