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Published on:December 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(4):1056-1061
Original Article | doi:10.5530/ijper.54.4.200

Antiangiogenic Potential of Levetiracetam by Blocking N-type Voltage Gated Calcium Channels

Authors and affiliation (s):

Chandana Kamili1,*, Hima Sowmya Kandoti2, Sunil Kumar Sarolu2, Suresh Merugu3, Abbulu Konde4

1Assistant Manager, Medical Writing, Tata Consultancy Services, Mumbai, INDIA.

2Department of Pharmacology, CMR College of Pharmacy, Hyderabad, Telangana, INDIA.

3Department of Research and Development, CMR College of Engineering and Technology, Hyderabad, Telangana, INDIA.

4Department of Pharmaceutics, CMR College of Pharmacy, Hyderabad, Telangana, INDIA.


Objectives: Angiogenesis is generation of micro vessels. These ion channels on endothelium play vital functions in cell proliferation, migration, cell volume expansion and in related angiogenesis. Our study aims to evaluate the antiangiogenic capability of Levetiracetam, a N-type voltage gated calcium channel blocker. Methods: Anti-angiogenic activity was evaluated by MTT cell viability assay, Morphological screening assay, Zebra fish caudal fin assay and Zebra fish embryo assay using different concentrations of levetiracetam, standard drug Bevacizumab and control. The parameters assessed were percentage viability in MTT, change in the cell shape or volume microscopically in morphological screening assay, percentage regeneration of amputated fin in caudal assay and percentage neovascularization in zebra fish embryo assay. Results: The results suggest that levetiracetam treatment significantly inhibited Hep G2 cells viability and cell volume expansion, which are the further steps in the endothelial cell proliferation. Arrest in neovascularization caused various phonotypical changes in the zebra fish embryo has been captured us by microscope. A significant decrease in fin regeneration was observed. Conclusion: The findings statistically validate the antiangiogenic potential of levetiracetam. The antiangiogenic property might be due to the blocking of N-type voltage gated calcium channels, which are largely located in the endothelial cells.

Key words: Angiogenesis, Levetiracetam, Calcium channels, Cell migration, Neovascularisation.



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