Investigation of Effect of Phytoconstituents Aloe Emodin and Quercetin on Bioavailability of Albendazole

Abstract:

Background/Aim: Albendazole is a drug with benzimidazole nucleus and is poorly absorbed from the gastrointestinal tract due to its low aqueous solubility. The objective of present work was to study the effect of two phytoconstituents aloe emodin and quercetin on the bioavailability of albendazole. Materials and Methods: Estimation was done through UV spectroscopy and HPLC analysis using different concentration of both the phytoconstituents. In-vivo study was conducted to investigate the pharmacokinetics and pharmacodynamic parameters. Results: Results revealed no change in T_max of albendazole and significant changes were observed in C_max values. The C_max was maximum i.e. 0.138 μg/ml when 20mg/kg of quercetin was added as compared to aloe-emodin. The increase in bioavailability of Albendazole was due to the presence of flavonoids in quercetin. The retention time of albendazole as estimated through HPLC chromatogram was found to be 3.2 minutes with acetonitrile and phosphate buffer as the mobile phase. However; albendazole was not detectable in 24 hr plasma sample and values of AUC were found to be zero in all the concentration of aloe emodin and quercetin. Conclusion: Thus, the phytoconstituents rich in flavonoids could be used to increase the bioavailability of poor soluble drugs.

Key words: Albendazole, Aloe emodin, Quercetin, Bioavailability, Pharmacokinetics.