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Published on:March 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(1s):s202-s208
Original Article | doi:10.5530/ijper.55.1s.51

Biogenic Synthesis of Silver Nanoparticles (AgNPs) using Solanum indicum Linn


Authors and affiliation (s):

Sadeq Khalid Alhag1,2, Hamed Ali Ghramah3,4, Lamya Ahmed Al-keridis5, Fahd Abdu Al-Mekhlafi6,7,*, Essam Hassan Ibrahim3,8,9, Khalid Ali Khan3,10

1Biology Department, College of Science and Arts, King Khalid University, Muhayl Asser, SAUDI ARABIA.

2Biology Department, College of Science, Ibb University, YEMEN.

3Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA.

4Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA.

5Department of Biology, Faculty of Science, Princess Nourah bint Abdulrahman University, Riyadh, SAUDI ARABIA.

6Bioproducts Research Chair, Department of Zoology, Faculty of Science, King Saud University, SAUDI ARABIA.

7Department of Agricultural Production, College of Agriculture and Veterinary Medicine, Thamar University, YEMEN.

8Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA.

9Blood Products Quality Control and Research Department, National Organization for Research and Control of Biologicals, Cairo, EGYPT.

10Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, SAUDI ARABIA.

Abstract:

Background: Synthesis of silver nanoparticles (AgNPs) using green plants is important for biocompatibility, reduced hazards, green policy and eco-friendliness. Materials and Methods: In this study, a leaf extract of Solanum indicum and associated AgNPs were used to examine larvicidal properties and other biological activities. AgNP forms were characterized with scanning electron- microscopy (SEM), Fourier Transform Infrared Radiation spectroscopy (FTIR) and UV-vis spectra. Larvicidal activity was assessed with Culex pipiens larvae using the World Health Organization method. Antibacterial potential of S. indicum leaf extract and AgNPs were evaluated with a well diffusion assay. In vitro impacts of S. indicum extract and AgNPs were studied using spleen cell propagation. Results: S. indicum ethanol extract and associated AgNPs show larvicidal activity against 4th instar of Cx. pipiens. Synthesized AgNPs were more toxic, with lower lethal concentration values, (LC50 = 47.181 ppm; LC90 = 243.776ppm) compared to plant extract (LC50 = 131.448 ppm; LC90 = 4397.528 ppm) after a twenty-four hour of exposure. Synthesized AgNPs also show significant impact Staphylococcus aureus, Proteus mirabilis, Escherichia coli and Shigella flexneri. Cytotoxic effects were demonstrated for S. indicum extract and AgNPs on normal spleen cells. The extract and associated AgNPs were also safe for red blood cells (RBCs) and failed to cause substantial elevation in serum liver enzymes. Conclusion: Leaf extracts of S. indicum and associated AgNPs may be efficient and eco-friendly for control of Cx. pipiens. Their antibacterial activity may be useful if care is taken to prevent cytotoxic effects.

Key words: Cytotoxic effect, Culex pipiens, Silver Nanoparticles, Antibacterial, Larvicidal, Solanum indicum.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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