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Published on:June 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(2s):s563-s571
Original Article | doi:10.5530/ijper.55.2s.128

Protective Effect of Boerhavia diffusa in Attenuating Pro-inflammatory Cytokines and Inhibition of Activated NF-κB-TNF-α-Nrf2 in Freund’s Adjuvant-induced Rheumatoid Arthritis


Authors and affiliation (s):

Ritu Karwasra1,*, Shivkant Sharma2, Nitin Sharma3, Kushagra Khanna4

1Department of Pharmacology, All India Institute of Medical Sciences (AIIMS), New Delhi, INDIA.

2Department of Pharmaceutical Sciences, Gurugram University, Gurugram, Haryana, INDIA.

3Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, Meerut, Uttar Pradesh, INDIA.

4Department of CEPIN, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, New Delhi, INDIA.

Abstract:

Background: Boerhavia diffusa is widely used in Asian and African countries in the treatment of inflammatory disorders in adjunct with other herbal formulations. The current research work was devised with an aim to examine the protective effect of hydroalcoholic root extract of Boerhavia diffusa in rheumatoid arthritis. Materials and Methods: Hydroalcoholic extract of plant at varying doses and reference drug (indomethacin 3mg/ kg), were administered daily for 21 days. The parameters such as joint diameter, level of antioxidant (GSH) and pro-oxidant (MDA) markers and histolopathological evaluation were performed. Immunohistochemical analysis was carried out to evaluate the effect of Boerhavia diffusa on pro-inflammatory (IL-1), anti-inflammatory (IL-10), pro-inflammatory TNF-α and its receptor, TNF-R1, angiogenesis (VEGF), Nrf-2 and NF-κB. Results: Findings of study discovered that there is dose dependent significant (p<0.05) reduction in inflammation by varying doses of Boerhavia diffusa along with the reduction (p<0.01) in levels of oxidant stress markers. Notable decline in inflammation and joint dysfunction was found in Boerhavia diffusa 200 mg/kg dose with significance of p<0.001. Conclusion: We concluded that the tuberous roots of Boerhavia diffusa show dose dependently attenuate paw edema, inflammation and reversed the bone damage through inhibition of activated pro-inflammatory mediators, Nrf-2 and specifically NF-κB-mediated production of cytokines.

Key words: Boerhavia diffusa, Rheumatoid Arthritis, NF-κB pathway, Angiogenesis, Inflamation.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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