Home | Articles
Published on:March 2023
Indian Journal of Pharmaceutical Education and Research, 2023; 57(1s):s22-s31
Original Article | doi:10.5530/ijper.57.1s.4

Production of L-tyrosinase from Novel Variants of Streptomyces cellulosae

Authors and affiliation (s):

G Sowjanya*, G Girijasankar

A.U. College of Pharmaceutical Sciences, Pharmaceutical Biotechnology Division, Andhra University, Visakhapatnam, Andhra Pradesh, INDIA.


Background: Recently, a lot of interest has been focused on the discovery of high L-tyrosinaseyielding microorganisms that catalyze the production of enantiomerically pure L-DOPA, which has been recognized as the conventional treatment for Parkinson's disease. Objectives: The current work primarily focused on isolating novel, promising tyrosinase-producing isolates and performing taxonomical characterization on them. Materials and Methods: Initially six various actinomycetes isolates were used in present study. Primary screening and secondary screening of selected isolates were performed. By evaluating the morphological, cultural, physiological, biochemical and molecular characterization, promising isolates were identified. Using 16S rRNA gene sequencing, the isolates' Phylogenetic investigations were conducted. Results: Streptomyces cellulosae variants, isolates PD18 and PD26 were identified to be the potential actinomycete isolates and their respective maximal tyrosinase yields were reported to be 21.20 IU/mL and 24.19 IU/mL. Conclusion: According to the observations of the experiment, SS (Soluble starch) medium with 1mg/mL copper sulphate was most suitable medium for tyrosinase production from our promising isolates PD18 and PD26. The maximum yield was noticed after 168 hr of incubation. This work emphasizes the significance of finding novel variants of Streptomyces cellulosae, which were first used to produce tyrosinase as well as promising sources for potentially bioactive substances like antimicrobial metabolites, biosurfactants and enzymes like amylase, lipase, cellulase, uricase, urease, β- galactosidase and L- asparaginase.

Keywords: Tyrosinase, L- tyrosine, L-DOPA, SS medium, copper sulphate, Streptomyces cellulosae.



Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the Latest Release of Journal Citation Reports (source: Web of Science Data).


Impact Factor® as reported in the 2021 Journal Citation Reports® (Clarivate Analytics, 2022): 0.843

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs