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Published on:March 2023
Indian Journal of Pharmaceutical Education and Research, 2023; 57(2):591-597
Original Article | doi:10.5530/ijper.57.2.72

Development and Validation of UV-spectrophotometric Method for the Estimation of Wintergreen Oil in Pharmaceutical Formulation


Authors and affiliation (s):

Deepali1, Vijaya G Joshi1,*, Ramachandra Setty S2, Geetha M1

1Department of Pharmaceutics, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.

2Department of Pharmacology, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.

Abstract:

Objectives: The study aimed to develop and validate a simple UV-spectrophotometric method for quantifying Wintergreen oil in micro/nanosponges. Materials and Methods: The method was developed using acetonitrile as a solvent system and validated for various parameters such as linearity, precision, repeatability, the Limit of Detection (LOD), and the Limit of Quantification (LOQ), and accuracy according to ICH guidelines. Results: The oil showed an absorption maximum at 237nm. Linearity between concentration and absorbance was established within a concentration range of 2 to 28 mcg/mL and showed a regression coefficient of 0.9746. The recovery of 97.14-107.82% and % RSD of less than 2% for repeatability, intraday, inter-day, and ruggedness revealed that the method is accurate and precise. The LOD and LOQ were determined as 0.103 mcg/mL and 0.312 mcg/mL respectively. Insignificant changes in absorption values with deliberate variation in absorption maximum indicate the method's robustness. The entrapment efficiency and loading capacity ranged between 88-97.82% and 3.085-24.35%. Conclusion: The validation results conclude that the method is simple, sensitive, precise, accurate, and robust. Hence, the method was used for quantifying wintergreen oil in micro/nanosponges. The procedure can further be adapted for analysing wintergreen oil in other pharmaceutical preparations and commercial products.

Keywords: Wintergreen oil, UV-spectrophotometry, Validation, Micro/nanosponges, Pharmaceutical formulation.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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