Home | Articles
Published on:27th Dec, 2014
Indian Journal of Pharmaceutical Education and Research, 2015; 49(2):126-133
Pharmaceutical Research | doi:10.5530/ijper.49.2.8

In Silico Antiurolithiatic Screening of Aerva lanata (L) Isolated constituents

Authors and affiliation (s):

Basavaraj M Dinnimath1 and Sunil S Jalalpure*2

1Department of pharmachemistry, KLEU’s college of pharmacy Belgaum-590010, Karnataka-India

2Department of pharmacognosy, KLEU’s college of pharmacy Belgaum-590010, Karnataka-India


Background: Traditionally known as Pashanabheda in India, Aerva lanata (L) belonging to the family Amaranthaceae, is widely available in Western ghats of India and used as Antiurolithiatic, astringent, diuretic, antimicrobial, anti-inflammatory, hepatoprotective drug by Indian traditional system of medicines. Materials and Method: In the present study this herb is subjected to extraction with hydro alcohol followed by fractionation with different solvents of varying polarities such as dichloromethane, ethyl acetate and n-butanol and screened for Antiurolithiatic activity using ethylene glycol induced male Wistar albino rats. Based on the results, the potent fractions were subjected to isolation of active constituents using column chromatography. Results: The isolated compounds from two fractions n-butanol and ethyl acetate were characterized by modern analytical techniques such as IR, HPTLC, NMR and LCMS as Quercetin and Betulin. Later these two compounds were studied for Antiurolithiatic activity by In silico technique by docking with a protein 2 ETE of Oxalate oxidase from PDB and the results indicated better regio-specificity with the enzyme. Conclusion: The two compounds isolated from potent fractions based on bioactivity guided fractionation were characterized as Quercetin and Betulin by modern analytical techniques. These two compounds were studied by in silico method and these two compounds have produced significant results which substantiate their claim of bioactivity. However in vivo study is needed to confirm the activity.

Key words: Aerva lanata (L), Pashanabheda, isolation and characterization, Antiurolithiatic agents, In silico study.



Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).


Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

User login

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs