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Published on:September 2023
Indian Journal of Pharmaceutical Education and Research, 2023; 57(4):1012-1020
Original Article | doi:10.5530/ijper.57.4.114

Development, Evaluation, and in vitro Anti-Acne Activity of Tretinoin Nanocrystals Gel

Authors and affiliation (s):

Pottathil Shinu1,*, Akash Sharma2, Anroop B. Nair3, Manish Kumar4, Shahzad Khan1, Abdulaziz K. Al Mouslem3, Mohamed A. Morsy3,5, Abhishek Tiwari6, Varsha Tiwari6, Beena Kumari7

1Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.

2Department of Pharmaceutics, M. M. College of Pharmacy, Maharishi Markandeshwar (Deemed to Be University), Ambala, Haryana, INDIA.

3Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.

4School of Pharmaceutical Sciences, CT University, Sidhwan Khurd, Ludhiana, Punjab,  INDIA.

5Department of Pharmacology, Faculty of Medicine, Minia University, El-Minia, EGYPT.

6Department of Pharmaceutical Sciences, Pharmacy Academy, IFTM University, Moradabad, Uttar Pradesh, INDIA.

7Department of Pharmaceutical Sciences, Indira Gandhi University, Meerpur, Rewari, Haryana  INDIA.


Background: Tretinoin is generally used to treat acne vulgaris and photoaging. The study aimed to develop a gel composition containing tretinoin nanocrystals and characterize and compare in vitro anti-acne activity. Materials and Methods: The anti-solvent precipitation approach was used to prepare tretinoin nanocrystals. The drug nanocrystals were optimized for parameters such as particle size, drug content, and drug release. The optimized nanocrystals (TN9) were incorporated into the gel system (TNG1-TNG5) and characterized for pharmaceutical properties. The selected formulation (TNG1) was tested for anti-acne activity using Propionibacterium acnes and Staphylococcus epidermidis and compared with Doxycycline HCl as standard. Results: Nanocrystals (TN9) showed the lowest particle size of 114.2 nm, the least value of polydispersity of 0.232, the highest drug content of 95.24%, and higher cumulative drug release of 97.2% in 10 hr. TEM and DSC images conformed to the nano-size range and crystalline nature of nanocrystals. The prepared gel composition indicates a clear, smooth, and homogeneous nature, good spreadability, suitable viscosity, pH, and drug content. The drug release from TNG1 was found to be significant (p < 0.0001) and was superior to the commercial gel. Results of anti-acne activity signify that the zone of inhibition exhibited by tretinoin nanocrystals was comparable with Doxycycline HCl (standard) for both microorganisms tested. Conclusion: These results demonstrated that the developed tretinoin nanocrystals could be a feasible approach for effective therapy in the treatment of acne.

Keywords: Tretinoin, Nanocrystals, Gel, Drug release, Anti-acne activity, Propionibacterium acnes, Staphylococcus epidermidis.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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