Home | Articles
Published on:9th Jan, 2015
Indian Journal of Pharmaceutical Education and Research, 2015; 49(3):231-239
Pharmaceutical Research | doi:10.5530/ijper.49.3.9

RAPD Based Assessment of Genetic Diversity of Adhatoda vasica Leaves from Different Sub- Continents of India


Authors and affiliation (s):

Madhukar Garg1, Ennus Tajuddin Tamboli1, Mhaveer singh1, Karishma Chester1, Malik Zainul Abdin2, Ruby Chandna2, Sayeed Ahmad1

1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, Hamdard Nagar, New Delhi -110062, India

2Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard University, Hamdard Nagar, New Delhi -110062, India

Abstract:

The present work assessed the genetic divergence amongst the accessions of vasaka collected from different sub-climatic zones of India by RAPD (Randomly Amplified Polymorphic DNA) using twenty random decamer primers (OPA 1-OPA 20) as the plant was found to enjoyed its therapeutic efficacy in Ayurvedic and traditional system of medicines. The dendrogram constructed for cluster analysis using an un-weighted pair group method with arithmetic means (UPGMA) grouped the accessions into 2 major clusters based on winboot. Out of the twenty random primers used for studying genetic divergence sixteen primers were found to be polymorphic. Out of 20 primers 3 were found to be 100% polymorphic generating a total of 313 amplification products with an average of 19.5 products per polymorphic primer. Genetic relationships among accessions were evaluated by generating a similarity matrix based on Jaccard's co-efficient ranging from 0.60 to 0.91. Results showed that both environmental and genetic factors were effective in observing variations. The degree of genetic variations detected among the accessions of vasaka suggested that RAPD approach seemed to be best suited for assessing with high accuracy the genetic relationships among distinct A. vasica accessions.

Key words: RAPD, Genetic diversity, Adhatoda vasica, variation, Polymorphism, Primer.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
18 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

User login

CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
8 + 12 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs