Anti-anaphylactic and Anti-inflammatory Activities of AFPA from the Root Bark of Plumeria acutifolia Poir.

Abstract:

In the present study, anti-anaphylactic, anti-inflammatory and membrane stabilizing property of alkaloid rich fraction from the root bark of Plumeria acutifolia (AFPA) were investigated in animal models. The anti-anaphylactic activity of AFPA (100, 250 and 500 mg/kg) was studied by using models such as passive cutaneous anaphylaxis, passive paw anaphylaxis and also investigated for its anti-inflammatory activity against carrageenan induced paw edema and cotton pellet granuloma in albino rats. A dose-dependent beneficial effect was observed on leakage of evans blue dye in skin challenged with antigen and on paw anaphylaxis induced by antiserum. The alkaloid rich fraction also exhibited significant (p<0.01) inhibition of rat paw edema and granuloma tissue formation, including significant protection of RBC against the haemolytic effect of hypotonic solution, an indication of membrane-stabilizing activity. Anti-anaphylactic activity of AFPA may be possibly due to inhibition of the release of various inflammatory mediators. Anti-inflammatory activity of AFPA may be related to the inhibition of the early phase and late phase of inflammatory events.

Keywords: Plumeria acutifolia, Alkaloid, Anti-anaphylactic, Anti-inflammatory