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Published on:15th May 2015
Indian Journal of Pharmaceutical Education and Research, 2015; 49(4):282-292
Pharmaceutical Research | doi:10.5530/ijper.49.4.6

Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy


Authors and affiliation (s):

Byoung-Joo Seo, Vivek Kumar Bajpai, Irfan Ahmad Rather and Yong-Ha Park*

Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712- 749, KOREA.

Abstract:

This study was aimed to partially purify the exopolysaccharide (EPS) from the culture of Lactobacillus plantarum YML009 using ethanol precipitation method with a yield of 260 mg/L. Analytical evaluationby Bradford and Phenolsulphuric methods revealed the presence of 2.2% and 68.1% total protein and total sugar contents in partially purified EPS, respectively. Further, to confirm the biological potential, the EPS was evaluated for its antioxidant activity in various scavenging models including DPPH and nitrite radicals as well as reducing power ability. The partially purified EPS (5-40 mg/mL) displayed considerable amount of antioxidant efficacy on scavenging DPPH and nitrite radicals by 44.73%, and 43.93%, respectively. Moreover, EPS showed potentreducing power capacity along with total phenolic content 18.96 μg/mg of GAE.

Key words: Antioxidant, Exopolysaccharide, Free radical scavenger, Lactobacillus plantarum YML009, Total phenolic content.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs