Home | Articles
Published on:December, 2015
Indian Journal of Pharmaceutical Education and Research, 2016; 50(1):109-115
Pharmaceutical Research | doi:10.5530/ijper.50.1.14

Chitooligosaccharides Attenuate Lipopolysaccharideinduced Inflammation and Apoptosis of Intestinal Epithelial Cells: Possible Involvement of TLR4/NF-κB Pathway


Authors and affiliation (s):

Yuanping Yang1, Qiaoyun Tong1*, Hesheng Luo2, Ruo Huang1 and Zhongyan Li1

1Institute of Digestive Disease, Three Gorges University, Department of Gastroenterology, Yichang Central People’s Hospital, Yichang 443003, China.

2Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China.

Abstract:

Marine-derived chitooligosaccharides (CHOS) are a new class of anti-inflammatory natural products characterized by its nontoxity, low-cost and small-molecule. But whether CHOS exert a protective action against inflammatory bowel diseases (IBDs) is not yet fully understood. This paper studied the effect of CHOS on the inflammation and apoptosis of intestinal epithelial cells (IECs) using lipopolysaccharides (LPS)-stimulated Caco-2 cells as an in vitro model of IBD. Caco-2 cells were pre-incubated with various concentrations of CHOS (0.25, 0.5 and 1.0 mg/ml) for 2 h prior to being co-stimulated or not with LPS at a concentration of 1μg/mL for 48h. Cell apoptosis was measured with Annexin V-FITC and propidium iodide (PI) assay by flow cytometry. The levels of proinflammatory mediators including tumor necrosis factor (TNF)-α, interleukin-8 and prostaglandin (PG) E2 were analyzed using Enzyme-linked Immunosorbent Assay. And the cell expression of toll-like receptor 4 (TLR4), nuclear factor kappa B (NF-κB), caspase-3, bcl-2, and cyclooxygenase (COX)-2 was examined by western blot. We observed that CHOS significantly and concentration-dependently inhibited the LPS-induced inflammatory response and apoptosis of IECs, which was evidenced by the reduction in the release of proinflammatory mediators TNF-α, PGE2, the expression of COX-2, apoptotic (Annexin V+/PI-) cell populations, the pro-apoptotic caspase-3 expression, and the enhancement of the expression level of anti-apoptotic bcl-2. And CHOS treatment significantly reduced the protein expressions of TLR4 and NF-κB in LPS-stimulated Caco-2 cells. Thus, the present study suggests the potential medical use of CHOS in the control of IBDs, which may be due to the downregulation of TLR4/NF-κB pathway.

Key words: Chitooligosaccharides, Inflammatory bowel diseases, Lipopolysaccharides, Intestinal epithelial cells.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
1 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

User login

CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
3 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs