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Published on:December 2015
Indian Journal of Pharmaceutical Education and Research, 2016; 50(2s):S21-S31
Pharmaceutical Research | doi:10.5530/ijper.50.2.14

Inhalable Cationic Niosomes of Curcumin Enhanced Drug Delivery and Apoptosis in Lung Cancer Cells


Authors and affiliation (s):

Kiran Jyoti1, Ravi Shankar Pandey2, Jitender Madan1*, Upendra Kumar Jain1

1Department of Pharmaceutics, Chandigarh College of Pharmacy, Mohali (Panjab) INDIA.

2Department of Pharmaceutical Biotechnology, SLT Institute of Pharmaceutical Sciences, Guru Ghasidas University, Bilaspur, INDIA.

Abstract:

Background: Curcumin induces apoptosis in non-small cell lung cancer cells. Hence, inhalable cationic niosomes of curcumin were developed to surmount the poor physicochemical and biopharmaceutical limitations for effective drug delivery in lung cancer cells. Methods: Curcumin loaded freeze-dried cationic small unilamellar niosomes (Cur-C-SUNS) were prepared using reverse phase evaporation method and characterized in vitro using spectral, analytical and biological techniques. Results: The nanovesicle size, encapsulation efficiency and zeta-potential of Cur-C-SUNS were measured to be 97.4±8.3 nm, 83.3±5.1% and +28.5±1.25 mV, significantly (P<0.05) higher than 83.8±7.2 nm, 78.8±4.5% and -3.02±0.64 mV of optimized freeze-dried Cur-SUNS. Cur-C-SUNS inhibited the A549 lung cancer cells proliferation at the IC50 of 3.1 μM, significantly (P<0.05) lower than 7.5 μM of Cur-SUNS and curcumin suspension (<32 μM). Consistently, Cur-C-SUNS induced greater extent of apoptosis in comparison to Cur-SUNS and curcumin suspension. In addition, Cur-C-SUNS accumulated significantly (P<0.05) higher concentration of curcumin, 14.3±2.1 μg in A549 cells, as compared to 9.5±1.5 μg and 1.3±0.2 μg deposited, respectively by Cur-SUNS and curcumin suspension. At last, in vitro cellular uptake illustrated higher endocytosis of Cur-C-SUNS as compared to Cur-SUNS due to electrostatic interaction between cationic nanovesicles and negatively charged plasma membrane of A549 cells. Conclusion: In conclusion, promising in vitro attributes of Cur-C-SUNS in lung cancer therapy warrant further in vivo tumor regression study to scale up the technology for clinical translation.

Key words: Curcumin, Inhalation, Cationic niosomes, Lung cancer cells, Cytotoxicity, Apoptosis, Cellular uptake.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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