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Published on:August 2016
Indian Journal of Pharmaceutical Education and Research, 2016; 50(3s):S218-S224
Original Article | doi:10.5530/ijper.50.3.32

The Comparison and Analysis of Two Extraction Methods for Polysaccharides in Psidium guajava L. Fruits


Authors and affiliation (s):

Michael Antony Samy Amutha Gnana Arasi1*, Manchineela Gopal Rao2, Janardanan Bagyalakshmi3

1Assistant Professor, Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamil Nadu, INDIA.

2Vice Principal and Head of the Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamil Nadu, INDIA.

3Associate Professor, Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamil Nadu, INDIA.

Abstract:

Introduction: Fruit polysaccharides, essential health factors in foods, are widely present in edible fruits. Several methods for isolation of polysaccharide are available. Microwave Assisted Extraction (MAE) method is extensively used for separation of polysaccharides. Only little information is available about Psidium guajava L. Fruit Polysaccharide (PFP) and its extraction methods. Objective: In this study, two extraction methods of polysaccharides in Psidium guajava L. fruit were compared and the best method was ascertained. Methods: Psidium guajava L. polysaccharides were extracted using water extraction with conventional heating and microwave assisted extraction method. The factors that influenced water extraction with conventional heating included temperature, water to plant material ratio, extraction time and solvent to liquid ratio. The factors that influenced MAE of polysaccharides extraction included microwave power, irradiation time and solvent to liquid ratio. Yield is considered as the independent variable in both the methods. Results: By the result of the single factorial method and orthogonal experiments, the optimal extracting conditions were selected and then the best extraction method was ascertained. The optimum extraction conditions of water extraction with conventional heating include, temperature 90oC, water to plant material ratio 1:1, extraction time 1 hour 30 min and solvent to liquid ratio 2:1. The results showed that the extraction yield of microwave method was higher under the optimal conditions of irradiation time 20 min, microwave power 200 W and 3:1 solvent to liquid ratio than the water extraction with conventional heating.

Key words: Guava, Polysaccharides, Extraction, Optimization, Taguchi orthogonal, Comparison.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs