Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF-Beta1

Abstract:

Transforming growth factor-β1 (TGF-β1) is a known potent inducer causing epithelial to mesenchymal transition(EMT) in some cancers. The present study was to evaluate the effect of hesperidin on EMT of non-small cell lung cancer (NSCLC) in vitro. In this study, A549 cells, a cellular line of non-small cell lung cancer, were used for evaluation of EMT induced by TGF-β1. MTT assay was used to evaluate the effect of hesperidin on cell viability with or without TGF-β1 co-culture. At the same time of TGF-β1 addition or after cellular morphological change by TGF-β1 stimulation, hesperidin was given to cells, respectively. Roundness of cells, E-cadherin, α-SMA, Col-I, MMP-9, TIMP-1 were evaluated EMT by TGF-β1 induction and effect of hesperidin on the transition. 40 and 80μM hesperidin showed significant inhibition on cellular viability without TGF-β1; coculture with TGF-β1, 40μM hesperidin showed significant inhibition on the viability but not showed significant inhibition after TGF-β1 stimulation. Whether co-culture with TGF-β1 or addition after TGF-β1, 40μM hesperidin showed inhibitory effect on epithelial to mesenchymal transition and inhibitory effect on extracellular matrix degradation of MMP-9. The above results suggest that hesperidin could be a potential candidate for inhibited migration of non-small cell lung cancer.

Key words: Hesperidin, Non-small cell lung cancer, Transforming growth factor-β1, Proliferation, Epithelial to mesenchymal transition.