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Published on:Oct 2016
Indian Journal of Pharmaceutical Education and Research, 2016; 50(4):583-590
Original Article | doi:10.5530/ijper.50.4.10

Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF-Beta1


Authors and affiliation (s):

Hai-bin Yu1, LI Li2, Zhou-xin Ren3, 4 *, Jun-ling Shen1

1The First Affiliated Hospital of Henan University of TCM, Zhengzhou 450000, China.

2The Hospital of Henan Academy Institute of Traditional Chinese Medicine, Henan Zhengzhou 450000, China.

3Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.

4Collaborative Innovation Center for Respiratory Disease Diagnosis And Treatment & Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.

Abstract:

Transforming growth factor-β1 (TGF-β1) is a known potent inducer causing epithelial to mesenchymal transition(EMT) in some cancers. The present study was to evaluate the effect of hesperidin on EMT of non-small cell lung cancer (NSCLC) in vitro. In this study, A549 cells, a cellular line of non-small cell lung cancer, were used for evaluation of EMT induced by TGF-β1. MTT assay was used to evaluate the effect of hesperidin on cell viability with or without TGF-β1 co-culture. At the same time of TGF-β1 addition or after cellular morphological change by TGF-β1 stimulation, hesperidin was given to cells, respectively. Roundness of cells, E-cadherin, α-SMA, Col-I, MMP-9, TIMP-1 were evaluated EMT by TGF-β1 induction and effect of hesperidin on the transition. 40 and 80μM hesperidin showed significant inhibition on cellular viability without TGF-β1; coculture with TGF-β1, 40μM hesperidin showed significant inhibition on the viability but not showed significant inhibition after TGF-β1 stimulation. Whether co-culture with TGF-β1 or addition after TGF-β1, 40μM hesperidin showed inhibitory effect on epithelial to mesenchymal transition and inhibitory effect on extracellular matrix degradation of MMP-9. The above results suggest that hesperidin could be a potential candidate for inhibited migration of non-small cell lung cancer.

Key words: Hesperidin, Non-small cell lung cancer, Transforming growth factor-β1, Proliferation, Epithelial to mesenchymal transition.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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