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Published on:Oct 2016
Indian Journal of Pharmaceutical Education and Research, 2016; 50(4):638-648
Original Article | doi:10.5530/ijper.50.4.16

Anti-inflammatory screening of ethanolic leaf extract of Vernonia arborea Buch. –Ham.in formalin induced albino wistar rats


Authors and affiliation (s):

Sriram Sridharan1*, Meenaa Venkatramani2, Kavitha Janakiraman2, Brindha Pemiah1, Sasi kumar Chinnagounder2

1Centre for Advanced Research in Indian System of Medicine (CARISM), SASTRA University, Thanjavur 613 401, Tamil Nadu, INDIA.

2Post Graduate and Research Department of Biotechnology, Nehru Memorial College (Autonomous), Puthanampatti, Tiruchirappalli, Tamil Nadu, INDIA.

Abstract:

Context: The therapeutic potential of the genus Vernonia has been well documented over the years. However the pharmacological potential of the plant Vernonia arborea (Asteraceae) has been relatively less explored. Objective: To investigate the antiinflammatory effect of the ethanol leaf extract of V. arborea against formalin induced inflammation in rats. Materials and methods: Formalin induced inflammation method was used to induce inflammation in rats.0.1 ml/kg body weight (b.w.) of formalin was administered to the rats via sub-plantar injection to induce inflammation. The rats were pretreated with ethanol leaf extract of V. arborea at concentrations of 100, 200 and 300 mg/kg b.w. once daily for 30 days. Indomethacin (25 mg/kg b.w.) was used as standard drug. Biochemical and hematological parameters along with levels of antioxidant enzymes were evaluated. Results: Administration of formalin reduced antioxidant enzyme levels, total RBC and Hb. Pretreatment with ethanol leaf extract of V.arborea at dose dependent levels significantly increased (p<0.05)the levels of the above parameters. A significant increase (p<0.05) in paw thickness, level of serum enzymes (Aspartate transaminase, Alanine transaminase, Alkaline phosphatase and Creatine kinase), Lipid peroxide and leucocytes was observed in the rats induced with formalin, while these levels were normalized on pretreatment with ethanol leaf extract of V.arborea. Conclusion: The results of the present investigation demonstrate the antioxidant and anti-inflammatory potential of the ethanol leaf extract of V.arborea. However, it is imperative to isolate and purify the active principles involved in the pharmacological potency of this plant and determine its mechanism of action.

Key words: Vernonia arborea, formalin induced inflammation, indomethacin.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs