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Published on:July 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(4):684-691
Original Article | doi:10.5530/ijper.51.4.101

Response Surface Methodology Optimization of Thebaine Biotransformation into Codeine and Morphine Using Bacillus sp. FAR


Authors and affiliation (s):

Nazanin Ataee1, Jamshid Fooladi2,*, Mohammad Hasan Namaei3, Fateme Mirzajani4

1Department of Microbiology, Faculty of Biological Science, Alzahra University, Vanak, Tehran, IRAN.

2Department of Biotechnology, Faculty of Biological Science, Alzahra University, Vanak, Tehran, IRAN.

3Birjand Infectious Diseases Research Center, Birjand University of Medical Sciences, Ghafary Ave., Birjand, IRAN.

4Departmrnt of Nanobiotechnology, Protein Research Center (PRC), Shahid Beheshti University, G.C., Tehran, IRAN.

Abstract:

Background: Codeine and morphine are important pharmaceuticals having analgesic characteristics. Aim: The current study used response surface methodology to optimize the biotransformation of thebaine into codeine and morphine using Bacillus sp. FAR. Methods: A central composite design was used to determine the optimal concentrations of buffer and thebaine, pH, time, temperature, and biomass. Improvement in transformation was monitored using HPLC-DAD. Results: The optimized conditions employed were pH 7.7 at 36°C for 30 h using a thebaine concentration of 80 μg/mL, 45 mM tris of buffer and 2.9% w/v biomass. The optimal conditions for codeine and morphine production were 2.995 and 1.113 μg, respectively. Conclusion: Optimization demonstrated that pH and biomass had the strongest effects on biotransformation.

Key words: Biotransformation, CCD, Codeine, Morphine, Thebaine, Bacillus.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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