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Published on:July 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(3s):s195-s199
Original Article | doi:10.5530/ijper.51.3s.11

Genetic Diversity of Local Endemic Teucrium leucophyllum Montbret & Aucher ex Bentham. (Lamiaceae) in Turkey


Authors and affiliation (s):

Emel Sözen1*, Muhip Hilooğlu1, Ali Kandemir2

1Department of Biology, Faculty of Science, Anadolu University, 26470, Eskisehir, TURKEY

2Department of Biology, Faculty of Science and Art, Erzincan University, 2400, Erzincan, TURKEY

Abstract:

Background and Objective: Teucrium leucophyllum is endemic plant species having considerable narrow distribution in Erzincan (Turkey) Region. The aim of this study was to determine genetic diversity levels of T. leucophyllum in order to obtain data for its conservation. Method: Genomic DNA was isolated from 81 plants using CTAB method. Genetic diversity in four natural populations of T. leucophyllum was investigated by ISSR markers. A binary matrix was produced by scoring each amplified fragment as present (1) or absent (0) from each individual. The matrix was used to produce an input file and analyzed using the software programs; POPGENE 1.32 and GenAlex. Results: Twenty primers amplified a total of 434 bright and discernible bands of 220–3000 bp, of which 431 bands (PPB: 99.31%) were polymorphic in 4 populations. The genetic diversity was high at the species level; the observed number of alleles (Na) was 1.993, the effective number of alleles (Ne) was 1.404, the Nei’s genetic diversity (H) was 0.263, and the Shannon’s information index (I) was 0.418. A high level of gene flow (Nm: 2.208) and low-moderate genetic differentiation (Gst: 0.184) were also observed. Analysis of molecular variance showed that 11% of the total genetic diversity resided among populations, while 89% within the populations. Conclusion: Our results indicated that the conservation efforts should aim to preserve the extant populations of this endangered species in its natural habitat. Seeds could be collected from Kayacik and Bagistas populations for ex situ conservation practices.

Key words: Genetic diversity, ISSR, PCR, Teucrium leucopyllum, Endemic plant.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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