Home | Articles
Published on:December 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(4s):s661-s666
Original Article | doi:10.5530/ijper.51.4s.96

Evaluation and Optimization of Effective-dose of Alloxan for Inducing Type-2 Diabetes Mellitus in Long Evans Rat


Authors and affiliation (s):

Shaikh Shahinur Rahman, Nilufar Yasmin, Abu Taher Mohammad Mizanur Rahman, Akteruz zaman, Mohammad Hafizur Rahman and Shakh Mohammad Abdur Rouf*

Department of Applied Nutrition and Food Technology, Islamic University, Kushtia, BANGLADESH.

Abstract:

Background: The optimal effective and safe dose of alloxan for inducing stable diabetes in rats has been long arguable. Lower doses can result in auto-reversion from diabetogenic state to normal state. On the other hand, higher doses cause toxicity and loss of experimental animals since it completely destroys the insulin-producing pancreatic β cells. Therefore, determination of effective as well as safe dose of alloxan to induce stable diabetes in experimental Long Evan rats is crucial for investigating on diabetes. Objective: To determine effective and safe dose of alloxan for inducing a stable diabetes mellitus in Long Evans Rats to facilitate availability of diabetic rats for studying on diabetes. Methods: Healthy adult Long Evans rats (80-156 g) were divided into eight groups and each group contains six rats. One group treated as a nondiabetic control (C) while the other seven groups (Group-2 to Group-8) were used as experimental diabetic groups. Different doses of alloxan (80, 100, 120, 130, 140, 150 and 160 mg/kg body weight) were injected through intraperitoneal routes in overnight fasting group-2 to group-8 rats respectively. Blood glucose levels were monitored before and after administering alloaxan dose (for 7 consecutive weeks) by using a blood glucose meter and test strips. Results: In the case of non-diabetic rats, the normal blood glucose levels were found between 7.75 to 10.8 mmol/L. On the other hand, a slow gradual increment of blood glucose levels were measured among the rats (group-2, group-3 and group-4) treated with low doses of alloxan (80, 100 and 120 mg/kg BW) until 5 weeks. However, the increased blood glucose levels were reverted back to its normal by the following two weeks. Group-5 and Group-6 rats treated with alloxan doses of 130 mg/kg and 140 mg/kg respectively stably increased blood glucose levels during the experimental periods. While rats in group-7 and group-8 (treated with 150 and 160 mg/kg BW) were expired by the experimental period might be due to severe alloxan toxicity. Group-6 rats treated with 140 mg/kg alloxan were more preferable because the diabetic level is much more stable and significant than group-5. Conclusion: The optimum effective dose of alloxan was found to be 140 mg/kg BW for induction of stable diabetes in Long Evan rats.

Key words: Alloxan, Diabetes mellitus, Long Evans Rat, induced diabetes, Toxicity.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
6 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

User login

CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
12 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs