Evaluation of Clerodendrum inerme (L.) Gaertn. on Burkitt’s Lymphoma Cancer

Abstract:

According to current census every year six million deaths occur in the world due to cancer. Hence, there is need of discovery and development of novel, safe and efficacious drugs for the treatment of various cancer. In the present study, an attempt has been made to explore the medicinal aspects of Clerodendrum inerme (L.) Gaertn.. Verbenaceae plant involving cytotoxic and antiproliferative potential as hydroalcoholic extract using methanol and water (70:30 V/V). Preliminary phytochemical investigation confirms the presence of the moiety in hydro-alcoholic extract and cytotoxic analysis using brine shrimp lethality assay, Daudi cell line culture, dye exclusion assay, MTT assay and animal model. The extract showed cytotoxicity below 100 ppm and LD₅₀ at 30 ppm level on brine shrimp lethality assay and on being subjected to MTT assay. A decrease in cell viability was observed at 213 μg/ml concentration and showing antitumor efficacy against Burkitt’s lymphoma cells as well tumor model in female mice which showing the increased life span, as compared to control group, when treated with C. inerme (200 and 400 mg/kg body weight) and doxorubicin (2.5 mg/kg body weight) exhibited significant effect on tumor parameters. Hence it can be stated that C. inerme has potent antitumor properties.

Key words: Clerodendrum inerme, Cytotoxicity, Antiproliferative , Lethality, MTT, Daudi