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Published on:January 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(2):241-247
Original Article | doi:10.5530/ijper.52.2.27

Evaluation of Clerodendrum inerme (L.) Gaertn. on Burkitt’s Lymphoma Cancer


Authors and affiliation (s):

Chouhan Mahendra Kumar1,2*, Hurkadale Pramod Jayadevappa1, Hegde Harsha Vasudev3

1Department of Pharmaceutical Biotechnology, College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, (Deemed-to-be University), Belagavi, Karnataka, INDIA.

2Dr. Prabhakar Kore Basic Science Research Center, KLE Academy of Higher Education and Research, (Deemed-to-be University), Belagavi, Karnataka, INDIA.

3ICMR- National Institute of Traditional Medicine (Formerly Regional Medical Research Center), Belagavi, Karnataka, INDIA.

Abstract:

According to current census every year six million deaths occur in the world due to cancer. Hence, there is need of discovery and development of novel, safe and efficacious drugs for the treatment of various cancer. In the present study, an attempt has been made to explore the medicinal aspects of Clerodendrum inerme (L.) Gaertn.. Verbenaceae plant involving cytotoxic and antiproliferative potential as hydroalcoholic extract using methanol and water (70:30 V/V). Preliminary phytochemical investigation confirms the presence of the moiety in hydro-alcoholic extract and cytotoxic analysis using brine shrimp lethality assay, Daudi cell line culture, dye exclusion assay, MTT assay and animal model. The extract showed cytotoxicity below 100 ppm and LD50 at 30 ppm level on brine shrimp lethality assay and on being subjected to MTT assay. A decrease in cell viability was observed at 213 μg/ml concentration and showing antitumor efficacy against Burkitt’s lymphoma cells as well tumor model in female mice which showing the increased life span, as compared to control group, when treated with C. inerme (200 and 400 mg/kg body weight) and doxorubicin (2.5 mg/kg body weight) exhibited significant effect on tumor parameters. Hence it can be stated that C. inerme has potent antitumor properties.

Key words: Clerodendrum inerme, Cytotoxicity, Antiproliferative , Lethality, MTT, Daudi

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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