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Published on:JANUARY 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(1):62-70
Original Article | doi:10.5530/ijper.52.1.7

Development and Evaluation of High Oxaliplatin Loaded CS-g-PNIPAAm Co-Polymeric Nanoparticles for Thermo and pH Responsive Delivery


Authors and affiliation (s):

Archana Sidagouda Patil*, Anand Panchakshari Gadad

Department of Pharmaceutics, KLE University College of Pharmacy Belagavi, KLE Academy of Higher Education and Research Belagavi-590010, Karnataka, INDIA.

Abstract:

Background: Chitosan-g-poly(N-isopropylacrylamide) (CS-g-PNIPAAm) co-polymer was reported as a much efficient drug carrier but it shows very low percentage of drug loading in to the nanoparticles. Objective: The objective of the present study was to develop highly loaded pH and thermo responsive CS-g-PNIPAAm co-polymeric nanoparticles for tumor specific oxaliplatin delivery. Methods: CS-g-PNIPAAm co-polymer was synthesized by surfactant free dispersion copolymerization method and characterized for its structure (FTIR1H NMR), morphology and lower critical solution temperature (LCST). Different drug loading approaches like direct drug loading during polymerization and self-assembly methods were used. In direct drug loading method the chitosan concentration was varied and in self assembly method the polymer ratio and sonication time were varied for study. Drug loaded nanoparticles were evaluated for particle size, zeta potential, percent drug loading efficiency, percent drug content and in-vitro drug release study. Results: It was observed that, self-assembly method give a high amount of oxaliplatin loaded nanoparticles. Further, in direct loading method, as the concentration of chitosan in co-polymer increases, the percent drug loading and drug release at above LCST of copolymer also increases. Nanoparticles prepared by self assembly method (F-5) showed the maximum drug release at above LCST (pH 7.2) and trace amount of drug release below LCST (pH 7.5), indicating thermo and pH responsive delivery of oxaliplatin. Conclusion: In conclusion, higher oxaliplatin loading can be achieved by using self-assembly method with sonication time 5 min and drug:polymer ratio of about 3:10 and oxaliplatin release can be controlled by varying chitosan concentration in co-polymer.

Key words: Oxaliplatin, Chitosan-g-poly(N-isopropylacrylamide), Self assembly method, Thermo and pH responsive delivery.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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