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Published on:August 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(4s):s133-s139
Original Article | doi:10.5530/ijper.52.4s.89

Detection of the Genotoxicity of Gentiana L. Extracts by Using RAPD-PCR and ISSR-PCR Techniques


Authors and affiliation (s):

İlham Eröz Poyraz1*, İsmail Poyraz2, H. Tuba Kıyan3, Nilgün Öztürk3, Serdar Erken4, Fatih Gülbağ4, M. Ercan Özzambak5

1Department of Pharmaceutical Botany, Anadolu University, 26470 Eskişehir, TURKEY.

2Department of Molecular Biology and Genetics, Bilecik Şeyh Edebali University, 11210 Bilecik, TURKEY.

3Department of Pharmacognosy, Anadolu University, 26470 Eskişehir, TURKEY.

4Republic of Turkey Ministry of Food, Agriculture and Livestock, Atatürk Horticultural Central Research Institute, Yalova, TURKEY.

5Department of Horticulture, Ege University, 35100 İzmir, TURKEY.

Abstract:

Backround: The RAPD- and ISSR-PCR techniques are offering an insight into the detecting the genotoxicity of Gentiana extracts. Objective: It is aimed with the present study, to detect the genotoxicity of methanol extracts of ten Turkish Gentiana L. taxa on germinated Allium cepa L. root tips. Methods: RAPD- and ISSR-PCR techniques were used for detection of genotoxicity of Gentiana extracts. Results: Four RAPD and three ISSR primers produced the reproducible polymorphic and monomorphic banding patterns among 10 RAPD and 10 ISSR primers for all DNA samples. It is not any serious alteration along with band intensity change, the disappearance of the bands, and appearance of the new bands in the band profiles amplified from the Gentiana extracts-treated genomic DNA sample of A. cepa. The most efficient results were obtained by RAPD-P9 and ISSR-1 primer among the seven productive primers. Discussion: It is not observed any variation in the RAPD- and ISSR-PCR band profiles in time and concentration-dependent manner. Conclusion: It is determined that the three different concentrations of Gentiana extracts did not interact with the A. cepa DNA.

Key words: Gentiana L., Genotoxicity, RAPD-PCR, ISSR-PCR.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs