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Published on:June 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(4):618-625
Original Article | doi:10.5530/ijper.52.4.72

Gastroprotective Effect of Hydro-Alcoholic Extract of Polygonum bistorta Lin Root in Indomethacin- Induced Gastric Ulcers in Sprague Dawley Rats


Authors and affiliation (s):

Mohammad Khushtar, Aijaz Ahmad, Md. Azizur Rahman*

Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh 226026, INDIA.

Abstract:

Objective: Gastroprotective effect of hydro-alcoholic extract of Polygonum bistorta Linn root (HEPB) was investigated in indomethacin-induced gastric ulcer in Sprague Dawley rats. Background: Polygonum bistorta has been used as hemostatic drug in Unani system of medicine due to its cold and dry temperament. Methods: The rats were grouped into six groups each consisting of five rats. Group-I, group-II, group-III, group-IV, group-V and group-VI rats received 1 mL/kg/day 1% carboxymethyl cellulose (CMC), 1 mL/kg/day 1% CMC, 500 mg/kg/day HEPB, 1000 mg/kg/day HEPB, 20 mg/kg/day ranitidine and 1000 mg/kg/day HEPB per oral (po) respectively for 10 days. Further, rats of all groups except group-I and group-VI were administered with 20 mg/kg b.wt indomethacin po on eleventh day. Then, rats were sacrificed, stomach was opened, and ulcer index was calculated. Mucus barrier and histopathology was determined. Rest of stomach was homogenized in buffer to evaluate antioxidant parameters thiobarbituric acid, catalase and superoxide dismutase (SOD). Results: HEPB in group-III and group-IV significantly (p<0.01) and dose dependently increased the levels of mucus, SOD and catalase while, decreased ulcer index and thiobarbituric acid reactive substances compared to that of ulcer control group-II. Histopathological findings showed that indomethacin treatment caused gastric ulcer while; HEPB treatment protected them from indomethacin-induced ulcer. Ulcer protection potency of HEPB 1000 mg/kg/day in group-IV was comparable to that of 20 mg/kg/day ranitidine in group-V. Conclusion: HEPB protected stomach from indomethacin-induced gastric ulcers in rats by prevention of induced muco-oxidative stress. Thus, HEPB possesses gastroprotective effect against indomethacin-induced gastric ulcers in rats.

Key words: Polygonum bistorta, Indomethacin, Oxidative stress, Ulcer index, PUD.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs