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Published on:November 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(4s2):S246-S251
Research Article | doi:10.5530/ijper.52.4s.104

Chorioallantoic Membrane (CAM) Assay of Different Extracts of Rhizome and Inflorescence of Heliconia rostrata


Authors and affiliation (s):

Moonmun Dhara*, Lopamudra Adhikari, Raja Majumder

School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar-751003, Odisha, INDIA.

Abstract:

Background: Rhizomes of Heliconia rostrata (Heliconiaceae) has antiophidic, in vitro antioxidant and antibacterial activity. Aim: Extracts of rhizome and inflorescence of H. rostratawere studied to test their angiogenesis and anti-angiogenesis effect using ex-ovo chorioallantoic membrane (CAM) assay. Methods: Dried defatted rhizomes of H. rostrata were macerated using ethanol, methanol and water to obtain rhizome ethanol extract (REE), rhizome methanol extract (RME) and rhizome aqueous extract (RAE), respectively. Similarly, defatted dried inflorescence of H. rostrata was macerated with water to obtain inflorescence aqueous extract (FAE). Dried extracts were used in 0.50, 1.00, 1.50, 2.00 and 2.50 mg dose applied in 3mm diameter filter paper for the CAM study. Pictorial as well as a semi-quantitative method was used to evaluate the antiangiogenic effect of the extracts in different doses. Changes before at 0h and after treatment at 4 h were compared. Results: The CAM treated with RAE and FAE showed anti-angiogenic effect at 2.50 mg dose per paper. While, CAM treated with REE and RME showed anti-angiogenic effect at 1.50 mg dose per paper. Semi-quantitative evaluation done by five different evaluators (blindly) suggested RME to be the strongest among the extracts in terms of the anti-angiogenic effect. No angiogenic effect is seen. Conclusion: But the higher doses at which the anti-angiogenic effect is seen suggests that Heliconia rostrata may be considered neither angiogenic nor anti-angiogenic.

Key words: Rhizome, Inflorescence, Chorioallantoic, Angiogenesis, Anti-angiogenesis.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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