Home | Articles
Published on:November 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(4s2):S229-S236
Research Article | doi:10.5530/ijper.52.4s.102

Impact of Surfactants on Formulation Parameters and in vitro cytotoxicity of Boswellic Acids Loaded Nanoparticles on Human Colon Cancer Cell Lines


Authors and affiliation (s):

Neeta Solanki, Harish Dureja*

Faculty of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, INDIA.

Abstract:

Objective: Boswellic acids extracted from the gum resin of plant Boswellia serrata are well reported for anti-inflammatory and anticancer activities. The Present investigation was aimed to optimize the surfactant concentration by formulating a series of Boswellic acids nanoparticles. Methods: Nanoparticles were prepared by nanoprecipitation technique using diverse ratios of non-ionic surfactants (Polyvinyl alcohol and Pluronic F-127). Effect of surfactant concentration was studied on size, polydispersity index, zeta potential, entrapment efficiency and loading capacity of nanoparticles. Transmission Electron microscopy was used to examine the effect of surfactant type on surface morphology of developed nanoparticles. In vitro cytotoxicity was evaluated by 3-(4, 5-dimethylthiazol- 2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay against colon cancer cells lines (HCT- 116). Results: Smallest particle size and polydispersity index (189.2 nm, 0.27), high entrapment (49.53±0.5) and loading capacity (23.64±0.43) with spherical shape was obtained in Pluronic F-127 nanoparticles in comparison to Polyvinyl alcohol nanoparticles. Pluronic F-127 nanoparticles showed higher cytotoxicity against colon cancer cell lines with IC50 value 21.3 μM having significant cytotoxic effects in comparison with Polyvinyl alcohol (IC50 value 23.2 μM) nanoparticles and Boswellia serrata extract (IC50 value 30.8 μM). Conclusion: It is concluded that the choice of surfactant is a critical parameter in the formulation of nanoparticles.

Key words: Boswellic acids, Nano precipitation, Surface morphology, MTT assay.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
2 + 2 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

User login

CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
3 + 13 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs