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Published on:February 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(2):310-315
Original Article | doi:10.5530/ijper.53.2.39

Method Development and Validation for the Estimation of Dothiepin Hydrochloride by Using RP-HPLC in PURE and Tablet Dosage Form


Authors and affiliation (s):

Prasanthi Thayi1*, Lakshman Rao Atmakuri2, Nandini Mandada3, Hemanth Mandava3, Bhuvaneswari Mandru3, Chaitanya Manne3

1Department of Pharmaceutical Analysis, Associate Professor, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.

2Department of Pharmaceutical Analysis, Professor and Principal, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.

3Student, V.V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.

Abstract:

Aim: A simple, sensitive, accurate and precise RP-HPLC method was developed for the determination of Dothiepin HCl (DTH) in pure and tablet dosage form. Methods: The method was developed by using Phenomenex C18 (250 X 4.6 mm, 5 μm) and the mobile phase composed of buffer (0.1M sodium acetate): acetonitrile in the ratio of 50:50 v/v. The buffer pH was adjusted to 2.8. The retention time for Dothiepin HCl was found to be 3.44 min. Linearity range for Dothiepin HCl was found to be 10-60 μg/mL and the regression equation was found to be y=14691x-12844. % RSD for intra- and inter-day precision was found to be 0.27% and 0.84%. Average mean recovery was found to be 99.94%. LOD and LOQ values obtained for Dothiepin HCl were found to be 0.825 μg/mL and 2.498 μg/mL respectively. Conclusion: The results are analysed statistically and are found to be satisfactory. Hence this method can be successfully employed for analysis of Dothiepin HCl in tablet dosage form.

Key words: Dothiepin HCl, RP-HPLC, Linearity, Dosage form, Precision.

 

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IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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