Home | Articles
Published on:March 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(2s):xx-xx
Research Article | doi:10.5530/ijper.53.2.x

In vitro Metabolism of Risperidone on Isolated Microsomes of Rat Liver: Metabolite Identification and Profiling by RP-HPLC and LC-MS Techniques


Authors and affiliation (s):

Prutha Shirodkar1,*, Harshal Chavan2, Rajendra Mogal3, Avinash Dhake1

1Department of pharmaceutical chemistry, S.M.B.T. College of pharmacy, Nashik, Maharashtra- 422 403, INDIA.

2Department of pharmaceutical chemistry, Sandip foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik,Maharashtra- 422 213, INDIA.

3Department of pharmaceutics, Sandip foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik, Maharashtra- 422 213,INDIA.

Abstract:

Objective: The objective of present research work is to study in vitro metabolismof risperidone on isolated microsomes of rat liver. Methods: Its biotransformationwas examined using isolated microsomes derived from rat livers. Their separationwas accomplished on Jasco RP-HPLC C-18 column (250mm x 4.6mm x 5μm) usingmethanol: 50mM Potassium dihydrogen phosphate (50:50 %v/v) mobile phase, detectedat a wavelength of 280nm. Results: In vitro metabolism of RIS was investigated usingthe cytochrome P450 (CYP450) enzymes supplemented with an NADPH- generatingsystem. Incubation of RIS with rat liver microsomes resulted in the formation of 2 majorhydroxy metabolites. Conclusion: Formation of all the metabolites is correlated withPhase I biotransformation CYP enzyme subfamilies. The method was extended toLC-MS for further characterization of the metabolites using standards and the probablemetabolites were proposed.

Key words: RIS, Microsomes, Cytochrome P450, Biotransformation, LC-MS.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
1 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

User login

CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
1 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs