1Department of Biochemistry, University of Tabuk, Tabuk, SAUDI ARABIA
2Department of Home Economics, Faculty of Specific Education, Kafr ElShaikh University, EGYPT
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Tabuk, Tabuk, SAUDI ARABIA
4Department of Biochemistry, Faculty of Pharmacy, Zagazig University, Zagazig, EGYPT
5Department of Biology, University of Tabuk, Tabuk, SAUDI ARABIA
6Department of Biology, Faculty of Science, Ibb University, Ibb, YEMEN
7Biotechnology Divisions, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, INDIA
8Department of Chemistry, Faculty of Sciences, Tanta University, Tanta, EGYPT
9Department of Chemistry, University of Tabuk, Tabuk, SAUDI ARABIA
Corresponding author.
Author Notes
Copyright and License information
ABSTRACT
Background and Objectives
Selenium is considered one of the essential components of many enzymes, particularly the antioxidant enzymes such as thioredoxin reductase. Vegetables, among other food sources, are common sources of selenium. In the present study, the green synthesis of Selenium nanoparticles (SeNPs) from Ocimum basilicum’ aerial part was carried out, as were the anticancer and hepatoprotective roles of selenium nanoparticles against liver carcinogen acrylamide-induced toxicity.
Materials and Methods
The classification of SeNPs was done by different spectroscopic methods, including UV-spectroscopy, FT-IR, XRD, and HR-TEM. The synthesized SeNPs were tested for their anticancer properties using MTT assays (the normal human liver fibroblast cell line (WISH) and Human liver cancer (HepG2), respectively).
Results
The histopathological studies indicated that the liver acrylamide induced in mice showed necrosis and damage in liver cells, but the treatment of mice with SeNPs, reduced the effect of acrylamide significantly.
Conclusion
The results indicated the protective effect of SeNPs on acrylamide-induced cells both in vitro and in vivo.