1Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, INDIA
2department of Pharmaceutical Chemistry, Kamalakshi Pandurangan College of Pharmacy, Tiruvannamalai, INDIA
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ABSTRACT
Aim
To carry out Synthesis and Evaluation of novel 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells.
Materials and Methods
Alzheimer’s Disease (AD) has become a serious public health concern as a result of people living longer than ever before. It is critically necessary to discover a way to halt and postpone the illness. The present study aims to determine if new synthetic analogue pyrazole derivatives may protect against toxicity caused by Aβ25-35 and its underlying mechanisms in neuroblastoma cells like SH-SY5Y. PyRx 0.9 software was used for molecular docking studies. The cells of SH-SY5Y were preincubated for 30 min with varying doses of the generated compounds (C1-C10) to induce neurotoxicity. They were then grown in Aβ25-35 (25 mol/L) for 48 hr. Cell viability was determined by MTT assay.
Results
Compounds C5 and C8 exhibited a better binding score -8.4k/cal compared to other analogues. Synthesized compounds C5 and C8 inhibited Aβ25-35-induced apoptosis in SH-SY5Y cells and protected neural cells from damage.
Conclusion
The MTT assay confirmed that compounds C5 and C8 significantly reduced Aβ25-35-induced toxicity among human neuroblastoma SH-SY5Y cells, demonstrating its neuroprotective properties.