Home | Articles
Published on:August 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(3):638-648
Original Article | doi:10.5530/ijper.55.3.135

Gel-forming Mucins in Oviductus Ranae Contribute to Swelling Capacity by iTRAQ Proteomics Analysis


Authors and affiliation (s):

He Zhang1#, Yuyao Zhang2#, Chunhui Yang1, Wenjie Su2, Daian Pan1, Daqing Zhao3, Li Jing4,*, Bin Qi2,*

1Research Center of Traditional Chinese Medicine, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, CHINA.

2College of Pharmacy, Changchun University of Chinese Medicine, Changchun, CHINA.

3Jilin Provincial Key Laboratory of BioMacromolecules of Chinese Medicine, Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, CHINA.

4Practice Innovations Center, Changchun University of Chinese Medicine, Changchun, CHINA.

#He Zhang and Yuyao Zhang contributed equally to this work.

Abstract:

Introduction: Oviductus Ranae (OR) is the dried oviduct of female Rana temporaria chensinensis David, which is one of the best-known and highly valued oriental foods and medicines in China. OR has a unique physiological phenomenon that is high swelling capacity. Objectives: We aimed to study which components are associated with high swelling capacity in OR. Materials and Methods: isobaric tags for relative and absolute quantification (iTRAQ) proteomic methods were used to identify differentially expressed proteins between the OR and three counterfeit oviducts, including Rana nigromaculata, Bufo bufogargarizans and Rana catesbeiana to explore the mechanism of swelling capacity. Results: We found that a total of 1220 proteins were identified from 2149 unique peptide sequences. Comparing with three counterfeit oviducts, 11 differentially expressed proteins were identified in OR, including 9 up-regulated proteins, such as mucins (MUC2, MUC5AC, MUC5AC-like, MUC5B), PREDICTED: a-kinase anchor protein 13-like (AKAP13-like), PREDICTED: IgGFc-binding protein (FcγBP), PREDICTED: IgGFcbinding protein-like (FcγBP-like), et al. and 2 down-regulated proteins. Bioinformatics results found that these up-regulated proteins were involved in biological processes, including metabolic process, cellular process, responses to stimulus and biological regulation. Conclusion: Mucins, AKAP13-like, FcγBP and FcγBP-like may act as a positive regulator for the innate immune response in oviduct of Chinese brown frog and exceptional swelling capacity of OR is closely related to gel-forming mucins. Taken together, our results provide the basis for further understanding of the unique physiological phenomenon of OR.

Key words: Oviductus Ranae, Swelling capacity, Gel-forming mucins, Counterfeit oviducts, Differentially expressed proteins.

 




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the Latest Release of Journal Citation Reports (source: Web of Science Data).

 

Impact Factor® as reported in the 2020 Journal Citation Reports® (Clarivate Analytics, 2021): 0.638

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs