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Published on:August 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(3):748-755
Original Article | doi:10.5530/ijper.55.3.147

Stimulation of Cell Mediated Immune Response by Protein Hydrolysate from Porphyra yezoensis

Authors and affiliation (s):

Kalkooru Lakshminarayanan Venkatraman, Azeemullah Azmathullah Syed, Alka Mehta*

Department of Integrative Biology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, INDIA.


Aim: To investigate the immunomodulatory effects of the protein hydrolysate from Porphyra yezoensis. Objectives: In this study, the in vitro immunomodulatory effects of protein hydrolysate of Porphyra yezoensis was studied by assay of phagocytosis and Nitric oxide production in RAW 264.7 macrophages and DTH (Delayed type hypersensitivity), NBT (Nitroblue tetrazolium) and Neutrophil adhesion assay in mice. Results: Hydrolysate treatment was found to be non-toxic in both in vitro and in vivo models. In vitro results suggested that hydrolysate could enhance the phagocytic activity and also inhibit the production of nitric oxide. Oral administration of hydrolysate (500 mg/kg) evoked an increase in the percent neutrophil adhesion to nylon fibers as well as potentiated the DTH reaction induced by SRBC (Sheep red blood cells). A phagocytic response was observed with significant changes in formation of formazan crystals. Conclusion: The results indicated that enzymatic hydrolysate from P. yezoensis could enhance the cell mediated immune response. Thus, the Nori protein hydrolysate could be explored as a possible natural immune stimulant to be used as nutraceuticals.

Key words: Porphyra yezoensis, Enzymatic hydrolysate, Immunomodulation, Macrophages Nitrobluetetrazolium, Delayed-type hypersensitivity, Phagocytic index.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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