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Published on:October 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(4):xx-xx
Original Article | doi:10.5530/ijper.54.4.X

Evaluation of iceA1 Gene Expression of Helicobacter pylori Risk Factor of Gastric Cancer in Transgenic Brinjal


Authors and affiliation (s):

Mohammad Javad Mehran1, Basaralu Yadurappa Sathish Kumar1,2,*, Nanjundappa Haraprasad3, Rambod Barzigar1, Bashasab Fakrudin4, Sayan Paul5,6

1JSS Research Foundation, SJCE Technical Campus, Mysore, Karnataka, INDIA.

2Department of Biotechnology, JSS College, Ooty Road, Mysore, Karnataka, INDIA.

3JSS Science and Technology University, SJCE, Technical Campus, Mysore, Karnataka, INDIA.

4Department of Biotechnology and Crop Improvement, College of Horticulture, University of Horticulture Sciences Campus, GKVK Post, Bengaluru, Karnataka, INDIA.

5Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, INDIA.

6Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, Karnataka, INDIA.

Abstract:

Background: The advancement of plant biotechnology improved crop production by revolutionizing plant science. Humans were commonly infected by Helicobacter pylori, and it was closely linked to stomach ulcers and cancer. In addition to traditional vaccines for H. pylori, transgenic plants have also been produced to produce its antigens as well as edible and non-edible parts that can produce an immune response after consumption. The protein present in H. pylori associated with virulence (iceA1) is believed to predispose to stomach cancer. Objectives: The current study was aimed to evaluate the iceA1 gene expression within brinjal plant to produce novel transgenic lines LC420461-B7, -B10, -B15, -B22 and -B27. Materials and Methods: In the present study, amplified iceA1 gene from H. pylori strain 26695 was transformed into callus of brinjal (from leaf explants) through Agrobacterium tumefacians (EHA105). pBI121 vector was used in constructing the plant expression vector, and the transgenics generated were further evaluated by quantitative Real-Time PCR and western blot analysis. Results: Out of the 46 plants obtained five of them were found to be positive for the iceA1 expression. Both real time and western blot confirmed of the presence of expressed gene iceA1 within the plant sample leaves. By studying transgenic brinjal, the study may result in an H.pylorivaccine candidate. As well, the data can be used by researchers to get valid scientific information.

Key words: Helicobacter pylori, Transgenic brinjal, iceA1, Agrobacterium, Callus induction, Real-time PCR.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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