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Published on:November 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(4):1048-1059
Original Article | doi:10.5530/ijper.55.4.205

Attenuation of Cardiomyopathy Induced in Sub-Chronic Exposure of Acrolein by Sulforaphane via Indirect PPARy Expression Promoter


Authors and affiliation (s):

Promise Madu Emeka1,*, Hairul-Islam Mohamed Ibrahim2, Mohamed Aly Morsy1,3, Ibrahim Abdulraham Alhaider1,4, Snawar Hussian5, Emad Abdelaziz Ahmed2,6

1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.

2Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, SAUDI ARABIA.

3Department of Pharmacology, Faculty of Medicine, Minia University, El-Minia, EGYPT.

4R&D, Saudi Food and Drug Authority, Riyadh, SAUDI ARABIA.

5Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.

6Lab of Molecular Physiology, Department of Zoology, Faculty of Science, Assiut University, Assiut, EGYPT

Abstract:

Sulforaphane (SPN) is reported to activate the Nrf2/Keap1 complex responsible for protein and gene expression promotion of various antioxidant enzymes. The present study examined the role of Nrf2 in modulating other signaling pathways involved in SPN’s attenuation of acrolein (ACL)-induced cardiomyopathy in rats. Forty-two rat was categorized into seven 4-week treatment groups: control, SPN, losartan (LTN), ACL, ACL+SPN, ACL+LTN, and ACL+SPN+LTN. Heart samples were harvested for analysis; cardiac oxidative and injury biomarker levels and histopathological examination were undertaken. PPARγ, Nrf2, NF-κB, COX-2, and CYP2E1 protein expressions were examined. Results show that SPN and SPN+LTN reduced GSH, catalase, and lipid peroxidation compared to the ACL-treated group. Also, levels of creatine kinase-MB, cardiac troponin, and caspase 3 induced by ACL were all attenuated. Altered cardiac tissue pathophysiology by ACL was alleviated. SPN+LTN significantly increased Nrf2 expression via PPARγ action but decreased NF-κB and COX-2 expressions. Also, ACLincreased CYP2E1 expression was significantly attenuated by the SPN+LTN combination. For the first time, it suggests that SPN+LTN might offer a better therapeutic alternative to ACL-induced cardiomyopathy by activating Nrf2 via PPARγ and reducing NF-κB/COX- 2/CYP2E1 expressions.

Key words: Sulforaphane, Losartan, Acrolein, Cardiomyopathy, Oxidative Stress, PPARγ; Nrf2.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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