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Published on:June 2022
Indian Journal of Pharmaceutical Education and Research, 2022; 56(3):816-821
Original Article | doi: 10.5530/ijper.56.3.133

Potential of Ocimum sanctum to Inhibit the Growth of Pseudomonas aeruginosa, a Disease-causing micro-organism


Authors and affiliation (s):

Raj Narayan Yadav1, Sunil Kayesth2, Jyoti Arora1, Manpreet Bhalla1, Kamal Kumar Gupta2, Mohd Shazad2, Ankita Dey3, Veeranoot Nissapatorn4, Ajoy Kumar Verma1,*, Ravindra Kumar Dewan5

1Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, (NITRD) Sri Aurobindo Marg, New Delhi, INDIA.
2Department of Zoology, Deshbandhu College, University of Delhi, Delhi, INDIA.
3Biostatistics Section, National Institute of Tuberculosis and Respiratory Diseases, (NITRD) Sri Aurobindo Marg, New Delhi, INDIA.
4School of Allied Health Sciences, Walailak University, THAILAND.
5Department of Thoracic Surgery and Surgical Anatomy, National Institute of Tuberculosis and Respiratory Diseases, (NITRD) Sri Aurobindo Marg, New Delhi, INDIA.

Abstract:

Aim and Objectives: Plants are a good source of secondary metabolites, which have huge potential to kill micro-organisms. The present study aimed to evaluate the antimicrobial activity of Ocimum sanctum ethanol extract against drug-resistant and drug-sensitive strains of Pseudomonas aeruginosa (P. aeruginosa). Materials and Methods: A total of 119 strains of P. aeruginosa, 92 multidrug-resistant (MDR) and 27 pan sensitive strains were included in the study, which were isolated from different human clinical samples. Ocimum sanctum crude leaves extract was prepared with the standard “cold extraction method”. The susceptibility agar dilution plates were made by mixing molten Mueller Hinton Agar (MHA) media with plant extract in different quantities to get the final extract concentration of 0.2%, 0.1% and 0.05%. These plates were used for determining the sensitive/ resistance nature of all strains of P. aeruginosa against plant extract. Results: Among 92 MDR strains in MHA plates with 0.05% of plant extract, 59 strains were sensitive and 33 were resistant. The same MDR strains in MHA plate with 0.1% of the extract showed somewhat similar trends, 61 sensitive and 31 resistant. Whereas with 0.2% of the extract, 67 strains were sensitive, 11 showed resistance while 14 showed discrete colonies. Among 27 pan sensitive strains at 0.05% concentration, there was complete growth inhibition in 23, while discrete colonies were observed in 4, though, at 0.1% and 0.2%, all strains were found as sensitive. Conclusion: Analysis of the present study showed that Ocimum sanctum extract possess compounds with antimicrobial properties against commonly used isolates of Pseudomonas aeruginosa strains.

Keywords: Antimicrobial activity, Ocimum sanctum, Tulsi, Pseudomonas aeruginosa, Plant extract, Drug discovery.

 

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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