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Published on:June 2022
Indian Journal of Pharmaceutical Education and Research, 2022; 56(3):732-739
Original Article | doi:10.5530/ijper.56.3.122

Binary Solvent-assisted Graphene Synthesis by Direct Exfoliation of Graphite Powder and its Cytotoxicity on SiHa Cell Line

Authors and affiliation (s):

Prabhat Kumar, Purnima Jain, Anjana Sarkar*

Department of Chemistry, Netaji Subhas University of Technology, New Delhi, INDIA.


A cost-effective mass production of pristine graphene nanosheet is still an important challenge to harness its maximum potential in medical sciences. Here, we have used a binary solvent mixture of low dielectric constant (1,2,4- trichlorobenzene,ε = 2.24 and benzylamine,ε = 4.6) to produce a high quantity (0.6 mg/ml) of graphene under ultrasonication without compromising its quality. Obtained sample were characterized through XRD, UV-Vis, Raman, FT-IR, TEM-SAED, and cytopotency was evaluated on SiHa cell line. IG/I2D < 1.0 (~ 0.39) was achieved on more than 250 μm2 area, indicating the uniformity and obtained ID/IG ratio was 1.21. No toxicity was found in the case of 24 hr on the SiHa cell line. EC50 computation was 84.90 μg /ml for 48 hr and 62.03 μg /ml for 72 hr. Exfoliated graphene remained stable in binary solvent even after 1 year. The proposed experiment and obtained data can be powerful tools to explore the synergic effect of binary solvent exfoliation in combination to establish graphene as a biomaterial for neuronal network, tissue engineering, drug delivery, and regenerative medicines, to name a few.

Keywords: Graphene, Nanosheet, Cytotoxicity, SiHa Cell line, Biomaterial.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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