The aim of current study was the application of validated TLC-densitometric method for identification and determination of Estradiol hemihydrate in dosage forms. The applied TLC conditions were: Silicagel G60F254 glass plates; mobile phase: chloroform : acetone = 90 : 10 v/v, migration distance of mobile phase: 120 mm, UV-detection at = 254 nm. All of the experimental results for the content of Estradiol hemihydrate correspond to the respective confidence interval: Estrofem table: 1.78 mg ÷ 2.12 mg; Femoston F1 table: 1.88 mg ÷ 2.2 mg; Femoston F2 table: 1.99 mg ÷ 2.19 mg; Trisequens T1 table: 1.78 mg ÷ 2.18 mg; Trisequens T2 table: 1.92 mg ÷ 2.12 mg; Trisequens T3 table: 0.97 mg ÷ 1.17 mg. The proposed validated TLC-densitometric method is appropriate for quality control of Estradiol hemihydrate in commercially available tablets.
Key words: Estradiol hemihydrate, TLC, Densitometry, Tablets, analysis, Determination.