Objective: Evaluation of the cytotoxic effect of different extracts of Orthosiphon pallidus Royle on MCF-7 cell lines, comparatively. Materials and Methods: The breast cell line MCF-7 was cultured in DMEM medium containing fetal bovine serum & antibiotics. The cells were exposed to different doses of all the ethanolic, aqueous and hydroalcoholic extracts and incubated for 24, 48 and 72 hrs respectively and further studied for MTT colorimetric test, SRB test, glutathione assay and cellular uptake assay. Results: The results showed hydroalcoholic extract had a dose and time-dependent anticancer effect on MCF-7 cancer cells after 72 hours (P<0.05). The study revealed that Orthosiphon pallidus Royle could inhibit the growth of cancerous cells. Conclusion: The ethanolic, aqueous and hydroalcoholic extracts of Orthosiphon pallidus Royle exhibited anticancer activity in a wide range in dose -dependent manner. The study concluded that Orthosiphon pallidus Royle shows cellular uptake and anti-proliferative activities.
Key words: Breast Cancer, Cytotoxic Effect, MCF-7 Cell Line, Fetal Bovine Serum, Glutathione’s.