1Faculty of Pharmacy, DIT University, Dehradun, INDIA
2Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, INDIA
3Amity Institute of Pharmacy, Amity University, Noida, INDIA
4Senior Consultant and Head, Indira IVF, Dehradun, INDIA
Corresponding author.
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ABSTRACT
Aim/Background
Throughout oogenesis and folliculogenesis, the follicular fluid’s composition alters physiologically to fit the needs of particular microenvironmental demands. This study’s main goal was to compare the effect of follicular fluid collected from endometriotic and non-endometriotic patients on systemic body functioning of female mice.
Materials and Methods
Both healthy and endometriotic participants’ follicular fluid was collected and pooled separately. Female Swiss albino mice were injected with 1 and 2 mL/kg/day endometrial fluid in the intraperitoneal region and monitored for 21 days. Change in body weight, hormonal profile, glucose profile and hematological profile was monitored and recorded on regular basis.
Results
On day 21, the blood glucose level increased from 100.2±0.96 mg/dL to 138.4±3.32 mg/dL. Endometriotic follicular fluid had a dose-dependently decreased serum estradiol from 28.80±0.37 to 27.00±1.0 ng/mL and progesterone form 24.17±0.7 to 1.72±0.21 pg/mL and a rise in testosterone levels from normal 3.95±0.81 nmol/mL to 9.4±0.92. It has elevated serum LH levels to approximately three times normal levels. In contrast, the serum FSH level decreased from 19.40±0.74 mIU/mL to 2.5±0.22. As a result, the LH to FSH ratio increased from 0.18±0.01 to 3.9±0.19. There was a dose-dependent rise in serum insulin level significantly (p <0.001) from normal 0.74±0.02 IU/mL to 1.63±0.05 and 2.09±0.1 respectively. In a similar manner, HOMA-IR also showed increase in insulin resistance from normal 0.17 to 0.57±0.02 and 0.71±0.04. HOMA-Beta normal level was 8.17±0.3 increased to 10.05±0.4 beta cell dysfunction. Nevertheless, QUICKI were both dosages dependently decreased insulin sensitivity from 1.31±0.07 dose dependently to 0.9±0.01and 0.83±0.02.
Conclusion
Female mice treated with endometriotic follicular fluid of endometriosis patients displayed pancreatic abnormality. It has been concluded that endometriotic patients’ follicular fluid not only has a localized effect but also contains elements that enter the systemic circulation, have negative effects, and may be connected to significant clinical symptoms of endometriotic condition.