1Department of Pharmaceutics, School of Pharmaceuticals and Population Health Informatics, Faculty of Pharmacy, DIT University, Dehradun, INDIA
2Department of Pharmacology, SGT College of Pharmacy, SGT University, Gurugram, INDIA
3Department of Pharmaceutics, Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management (SPPSPTM), NMIMS University, Mumbai, INDIA
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ABSTRACT
Background
In current research, Zinc Nanoparticles (ZnNPs) were synthesized using green synthesis technology. It is the best method which is eco-friendly and cost-effective. The extract of fungus Cordyceps militaris was used for this purpose, which was followed by muffle furnace assisted synthesis of zinc nanoparticles.
Objectives
The main objective of this research work was to assess the anticholinesterase and antioxidant activity mediated Anti-Alzheimer activity of Cordyceps militaris and zinc nanoparticles prepared by green synthesis process.
Materials and Methods
The zinc nanoparticles were prepared by green synthesis process using extract of fungus Cordyceps militaris. Characterization of prepared nanoparticles were done by the Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), EDX and Fourier Transform Infrared (FT-IR) spectroscopy for shape and size, Surface Plasmon Resonance (SPR), functional groups, crystallinity. It was observed that SPR peak were formed 375 nm, which was determined through UV-visible Spectroscopy.
Results
SEM and TEM photomicrograph shown particles were formed spherical in the shape with smooth surface having 37.09 nm average particle sizes. SPR for nanoparticles was found at 300 nm. X-ray diffraction analysis confirm crystalline shaped of prepared nanoparticles. Moreover, during the synthesis of nanoparticles, the colour of suspension changed from dark yellow to colourless with development of cloudiness in solution. The different functional groups involved in nanoparticle stabilization were determined using FTIR Spectroscopy.
Conclusion
Furthermore, the Alzheimer activity of plant extracts and ZnNPs have been carried out using the Ellman technique through cholinesterase with small modification. The IC50 value in anticholinesterase inhibitory activity of zinc nanoparticles was found 83.16237 μg/mL while IC50 value of donepezil was found 51.38714 μg/mL which was taken as standard. It was concluded that the result indicated the promising inhibitory potential of cholinesterase activity. The antioxidant assay results with percentage inhibition 127.0308 μg/mL showed the most effective antioxidant property source than aqueous extract of Cordyceps militaris.