Published in: Indian Journal of Pharmaceutical Education and Research, 2025; 59(1): 94-100. Published online: 20 December 2024DOI: 10.5530/ijper.20255575
ABSTRACT
Aim
A sensitive and accurate RP-HPLC method was developed and validated for the quantitative measurement of Ceftriaxone sodium (CEF) in pharmaceutical dosage forms and bulk medicine.
Materials and Methods
Ceftriaxone sodium was separated on a 245 nm photodiode array detector using a Waters XTerra RP-18 (5 μm 250×4.6 mm internal diameter) column.
Results
The method generated an excellent linear response in the concentration range of 0.2-20 μg/mL with remarkable precision of 0.16-0.7% and accuracy in the percent recovery range of 99.88-99.97%.
Conclusion
We find the reverse phase HPLC technique to be highly sensitive, accurate, precise and user-friendly and hence recommend it. Therefore, it could be useful in a quality control lab.
Keywords: HPLC, Ceftriaxone, Bulk, Pharmaceutical, Dosage