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    Vol 59, Issue 1, 2025

    Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage

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    Author informationCitations

    Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, INDIA

    Corresponding author.

    Correspondence Dr. BasavarajHiremath Assistant Professor, Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad-585228, Kalaburagi, Karnataka, INDIA. Email: drbhiremath25@gmail.com

    Author Notes

    October 30, 2023; January 23, 2024; October 19, 2024.

    Copyright and License information

    Copyright Copyright ©2025 IJPER
    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
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    Published in: Indian Journal of Pharmaceutical Education and Research, 2025; 59(1): 94-100. Published online: 20 December 2024DOI: 10.5530/ijper.20255575

    ABSTRACT

    Aim

    A sensitive and accurate RP-HPLC method was developed and validated for the quantitative measurement of Ceftriaxone sodium (CEF) in pharmaceutical dosage forms and bulk medicine.

    Materials and Methods

    Ceftriaxone sodium was separated on a 245 nm photodiode array detector using a Waters XTerra RP-18 (5 μm 250×4.6 mm internal diameter) column.

    Results

    The method generated an excellent linear response in the concentration range of 0.2-20 μg/mL with remarkable precision of 0.16-0.7% and accuracy in the percent recovery range of 99.88-99.97%.

    Conclusion

    We find the reverse phase HPLC technique to be highly sensitive, accurate, precise and user-friendly and hence recommend it. Therefore, it could be useful in a quality control lab.

    Keywords: HPLC, Ceftriaxone, Bulk, Pharmaceutical, Dosage

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